Luminescent probes for ultrasensitive detection of nucleic acids.
Luminescent probes for ultrasensitive detection of nucleic acids.
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DOI：
10.1021/bc900403n
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发表时间：
2010-02-17
影响因子：
4.7
通讯作者：
Mustaev A
中科院分区：
文献类型：
--
作者：
Krasnoperov LN;Marras SA;Kozlov M;Wirpsza L;Mustaev A
合成了具有增强亮度和金属保留能力的基于镧系元素的发光标记的新型氨基反应衍生物，并用于通过分子信标检测互补DNA寡核苷酸。探针与靶标杂交时发生的发光信号的时间分辨采集能够避免短寿命的背景荧光，显着提高检测灵敏度（小于 1 pM）。该值比传统的基于荧光的分子信标所达到的水平敏感约50至100倍，并且比先前报道的其他基于镧系元素的杂交探针敏感10至60倍。这些新型发光标记应显着增强所有类型核酸杂交探针的灵敏度，并可显着提高用于各种生物应用的其他生物聚合物和小化合物的检测限。
Novel amino-reactive derivatives of lanthanide-based luminescent labels of enhanced brightness and metal retention were synthesized and used for the detection of complementary DNA oligonucleotides by molecular beacons. Time-resolved acquisition of the luminescent signal that occurs upon hybridization of the probe to the target enabled the avoidance of short-lived background fluorescence, markedly enhancing the sensitivity of detection, which was less than 1 pM. This value is about 50 to 100 times more sensitive than the level achieved with conventional fluorescence-based molecular beacons, and is 10 to 60 times more sensitive than previously reported for other lanthanide-based hybridization probes. These novel luminescent labels should significantly enhance the sensitivity of all type of nucleic acid hybridization probes, and could dramatically improve the detection limit of other biopolymers and small compounds that are used in a variety of biological applications.
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通讯作者：
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