Direct visualization of membrane architecture of myelinating cells in transgenic mice expressing membrane-anchored EGFP.
Direct visualization of membrane architecture of myelinating cells in transgenic mice expressing membrane-anchored EGFP.
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DOI：
10.1002/dvg.22751
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发表时间：
2014-04
期刊：
影响因子：
1.5
通讯作者：
Lu, Q. Richard
中科院分区：
文献类型：
--
作者：
Deng, Yaqi;Kim, BongWoo;He, Xuelian;Kim, Sunja;Lu, Changqing;Wang, Haibo;Cho, Ssang-Goo;Hou, Yiping;Li, Jianrong;Zhao, Xianghui;Lu, Q. Richard
关键词：
髓鞘形成是一个复杂的过程，涉及质膜结构和髓鞘与轴突相互作用的实质性和动态变化。在中枢神经系统（CNS）中，少突胶质细胞的高度分叉的突起使轴突接触，然后外推包裹轴突并形成多层致密的髓鞘。目前，髓鞘形成细胞对髓鞘组装和轴突选择的调控机制还不完全清楚。在这里，我们产生了一个转基因小鼠线表达髓鞘细胞中的膜锚定的绿色荧光蛋白（mEGFP），这使得在神经系统中的髓鞘形成和细胞行为的细节的实时成像。mEGFP表达由在髓鞘形成细胞谱系中表达的2′-3′-环核苷酸3′-磷酸二酯酶（CNP）的启动子驱动。稳健的mEGFP信号出现在CNS中的少突胶质细胞和外周神经系统（PNS）中的许旺细胞的膜过程中，其中mEGFP表达限定了成年坐骨神经中髓鞘和施密特-兰特曼切口的内层。此外，mEGFP表达可用于追踪毒素诱导的脱髓鞘动物模型中脱髓鞘损伤后的髓鞘再生程度。两者合计，在新的转基因株系中的膜锚定的mEGFP表达将有助于直接可视化的动态髓鞘膜的形成和组装过程中的发展和过程重塑过程中髓鞘再生后的各种脱髓鞘损伤。
Myelinogenesis is a complex process that involves substantial and dynamic changes in plasma membrane architecture and myelin interaction with axons. Highly ramified processes of oligodendrocytes in the central nervous system (CNS) make axonal contact and then extrapolate to wrap around axons and form multilayer compact myelin sheathes. Currently, the mechanisms governing myelin sheath assembly and axon selection by myelinating cells are not fully understood. Here, we generated a transgenic mouse line expressing the membrane-anchored green fluorescent protein (mEGFP) in myelinating cells, which allow live imaging of details of myelinogenesis and cellular behaviors in the nervous systems. mEGFP expression is driven by the promoter of 2′-3′-cyclic nucleotide 3′-phosphodiesterase (CNP) that is expressed in the myelinating cell lineage. Robust mEGFP signals appear in the membrane processes of oligodendrocytes in the CNS and Schwann cells in the peripheral nervous system (PNS), wherein mEGFP expression defines the inner layers of myelin sheaths and Schmidt-Lanterman incisures in adult sciatic nerves. In addition, mEGFP expression can be used to track the extent of remyelination after demyelinating injury in a toxin-induced demyelination animal model. Taken together, the membrane-anchored mEGFP expression in the new transgenic line would facilitate direct visualization of dynamic myelin membrane formation and assembly during development and process remodeling during remyelination after various demyelinating injuries.
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影响因子：
2.9
作者：
Toma, Jeremy S.;McPhail, Lowell T.;Ramer, Matt S.
通讯作者：
Ramer, Matt S.
影响因子：
16.2
作者：
Lu, QR;Yuk, DI;Rowitch, DH
通讯作者：
Rowitch, DH
影响因子：
16.2
作者：
Zhou, Q;Wang, SL;Anderson, DJ
通讯作者：
Anderson, DJ
DOI：
10.1073/pnas.1117943109
发表时间：
2012-10-09
影响因子：
11.1
作者：
Miller, Daniel J.;Duka, Tetyana;Sherwood, Chet C.
通讯作者：
Sherwood, Chet C.
Olig2 将染色质重塑剂靶向增强剂以启动少突胶质细胞分化
影响因子：
64.5
作者：
Yu Y;Chen Y;Kim B;Wang H;Zhao C;He X;Liu L;Liu W;Wu LM;Mao M;Chan JR;Wu J;Lu QR
通讯作者：
Lu QR