Dual effect of the wheat Ph1 locus on chromosome synapsis and crossover.
Dual effect of the wheat Ph1 locus on chromosome synapsis and crossover.
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DOI：
10.1007/s00412-017-0630-0
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发表时间：
2017-12
期刊：
影响因子：
1.6
通讯作者：
Moore G
中科院分区：
文献类型：
--
作者：
Martín AC;Rey MD;Shaw P;Moore G
异源多倍体必须具有促进同源物之间突触和交叉（CO）的机制，优先于同源物（相关染色体），以确保成功的减数分裂。在六倍体小麦中，Ph1 基因座对这些过程的控制具有重大影响。研究缺乏 Ph1 的小麦突变体提供了探索潜在机制的机会。最近，有人提出 Ph1 在减数分裂过程中稳定小麦，既通过在减数分裂早期促进同源物突触，又防止突触同源物上的 MLH1 位点在减数分裂后期变成 CO。在这里，我们探讨了这两种效应，并首先证明，无论Ph1是否存在，在端粒花束阶段，同源体之间的突触都不会发生，只有同源突触发生在该阶段。此外，在缺乏Ph1的小麦中，整体突触相对于端粒花束而言被延迟，在花束阶段之后发生更多的突触，此时同源突触也是可能的。其次，我们表明，在缺乏 Ph1 的情况下，我们可以通过改变环境生长条件来增加进展为 CO 的 MLH1 位点的数量；我们发现，土壤中较高的养分水平或较低的温度会增加同系物和同系物CO的水平。这些观察结果表明有机会在育种计划中改善 Ph1 小麦突变体的利用。本文的在线版本 (doi:10.1007/s00412-017-0630-0) 包含补充材料，可供授权用户使用。
Allopolyploids must possess a mechanism for facilitating synapsis and crossover (CO) between homologues, in preference to homoeologues (related chromosomes), to ensure successful meiosis. In hexaploid wheat, the Ph1 locus has a major effect on the control of these processes. Studying a wheat mutant lacking Ph1 provides an opportunity to explore the underlying mechanisms. Recently, it was proposed that Ph1 stabilises wheat during meiosis, both by promoting homologue synapsis during early meiosis and preventing MLH1 sites on synapsed homoeologues from becoming COs later in meiosis. Here, we explore these two effects and demonstrate firstly that whether or not Ph1 is present, synapsis between homoeologues does not take place during the telomere bouquet stage, with only homologous synapsis taking place during this stage. Furthermore, in wheat lacking Ph1, overall synapsis is delayed with respect to the telomere bouquet, with more synapsis occurring after the bouquet stage, when homoeologous synapsis is also possible. Secondly, we show that in the absence of Ph1, we can increase the number of MLH1 sites progressing to COs by altering environmental growing conditions; we show that higher nutrient levels in the soil or lower temperatures increase the level of both homologue and homoeologue COs. These observations suggest opportunities to improve the exploitation of the Ph1 wheat mutant in breeding programmes. The online version of this article (doi:10.1007/s00412-017-0630-0) contains supplementary material, which is available to authorized users.
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影响因子：
4.2
作者：
COX, AV;BENNETT, ST;BENNETT, MD
通讯作者：
BENNETT, MD
影响因子：
56.9
作者：
Borde, V;Goldman, ASH;Lichten, M
通讯作者：
Lichten, M
DOI：
10.1073/pnas.92.14.6645
发表时间：
1995-07-03
影响因子：
11.1
作者：
DUBCOVSKY, J;LUO, MC;DVORAK, J
通讯作者：
DVORAK, J
DOI：
10.1073/pnas.0608936104
发表时间：
2007-03-06
影响因子：
11.1
作者：
Francis, Kirk E.;Lam, Sandy Y.;Copenhaver, Gregory P.
通讯作者：
Copenhaver, Gregory P.
影响因子：
7.5
作者：
Klutstein M;Cooper JP
通讯作者：
Cooper JP