Application of a Novel Anti-Adhesive Membrane, E8002, in a Rat Laminectomy Model.
Application of a Novel Anti-Adhesive Membrane, E8002, in a Rat Laminectomy Model.
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DOI：
10.3390/ijms19051513
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发表时间：
2018-05-18
影响因子：
5.6
通讯作者：
Uchikado H
中科院分区：
文献类型：
--
作者：
Kikuchi K;Setoyama K;Terashi T;Sumizono M;Tancharoen S;Otsuka S;Takada S;Nakanishi K;Ueda K;Sakakima H;Kawahara KI;Maruyama I;Hattori G;Morioka M;Tanaka E;Uchikado H
脊柱手术后的神经性疼痛，即所谓的腰椎手术失败综合征，是一种常见的并发症。疼痛的原因之一是疤痕组织形成，观察到术后硬膜外粘连。这些粘连可能会压迫周围的脊神经，导致疼痛，即使在成功的脊柱手术后。E8002是防粘膜。在日本，目前正在接受腹部手术的患者中进行E8002的临床试验。然而，尚未在脊柱手术中对E8002进行动物实验。我们在大鼠椎板切除术模型中评估了E8002的抗粘连作用。硬脑膜物质用E8002膜覆盖或不覆盖作为对照。术后6周比较神经学评价和组织病理学结果。苏木精-伊红和醛复红-Masson Goldner染色进行组织学分析。在椎板切除部位的中间和边缘选择三个评估区域，计数成纤维细胞和炎性细胞的数量。盲态组织病理学评价显示，与对照组相比，E8002组的粘连和瘢痕形成减少。与对照组相比，E8002组的成纤维细胞和炎性细胞数量显著减少。目前的结果表明，E8002可以防止椎板切除术后硬膜外瘢痕粘连。
Neuropathic pain after spinal surgery, so-called failed back surgery syndrome, is a frequently observed common complication. One cause of the pain is scar tissue formation, observed as post-surgical epidural adhesions. These adhesions may compress surrounding spinal nerves, resulting in pain, even after successful spinal surgery. E8002 is an anti-adhesive membrane. In Japan, a clinical trial of E8002 is currently ongoing in patients undergoing abdominal surgery. However, animal experiments have not been performed for E8002 in spinal surgery. We assessed the anti-adhesive effect of E8002 in a rat laminectomy model. The dura matter was covered with an E8002 membrane or left uncovered as a control. Neurological evaluations and histopathological findings were compared at six weeks postoperatively. Histopathological analyses were performed by hematoxylin–eosin and aldehyde fuchsin-Masson Goldner staining. Three assessment areas were selected at the middle and margins of the laminectomy sites, and the numbers of fibroblasts and inflammatory cells were counted. Blinded histopathological evaluation revealed that adhesions and scar formation were reduced in the E8002 group compared with the control group. The E8002 group had significantly lower numbers of fibroblasts and inflammatory cells than the control group. The present results indicate that E8002 can prevent epidural scar adhesions after laminectomy.
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影响因子：
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作者：
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