The changes of gut microbiota after acute myocardial infarction in rats.
The changes of gut microbiota after acute myocardial infarction in rats.
复制标题
大鼠急性心肌梗死后肠道菌群的变化
DOI：
10.1371/journal.pone.0180717
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Cao CF
中科院分区：
文献类型：
--
作者：
Wu ZX;Li SF;Chen H;Song JX;Gao YF;Zhang F;Cao CF
最近的研究表明，肠道微生物群参与了冠状动脉疾病的发展。然而，急性心肌梗死（AMI）后肠道菌群的变化仍不清楚。本研究将66只雄性Wistar大鼠随机分为对照组、AMI组和SHAM组。对照组（n = 6）麻醉后处死。结扎冠状动脉左前降支建立急性心肌梗死模型。分别于术后12 h、1 d、3 d、7 d和14 d处死AMI组和SHAM组大鼠。通过16S rDNA高通量测序分析肠道微生物群。通过组织病理学、透射电镜和免疫组化染色观察肠屏障损伤情况。AMI后7d，AMI组肠道菌群丰富度显著高于SHAM组（P<0.05）。未加权UniFrac距离的主坐标分析显示AMI和SHAM组在7 d时的微生物差异。肠屏障损害在AMI后7d最为明显。进一步鉴定AMI组和SHAM组在第7天的微生物差异。术后7 d，AMI组血清中协同菌门、螺旋菌门、毛螺菌科、合成营养单胞菌科和Tissierella Soehngenia属的丰度均高于SHAM组（q<0.05）。本研究显示AMI后第7天肠道菌群的变化与肠屏障功能受损有关。我们还确定了贡献最大的微生物。
Recent studies suggested that gut microbiota was involved in the development of coronary artery disease. However, the changes of gut microbiota following acute myocardial infarction (AMI) remain unknown. In this study, a total of 66 male Wistar rats were randomly divided into control, AMI and SHAM groups. The controls (n = 6) were sacrificed after anesthesia. The AMI model was built by ligation of left anterior descending coronary artery. The rats of AMI and SHAM groups were sacrificed at 12 h, 1 d, 3 d, 7 d and 14 d post-operation respectively. Gut microbiota was analyzed by 16S rDNA high throughput sequencing. The gut barrier injuries were evaluated through histopathology, transmission electron microscope and immunohistochemical staining. The richness of gut microbiota was significantly higher in AMI group than SHAM group at 7 d after AMI (P<0.05). Principal coordinate analysis with unweighted UniFrac distances revealed microbial differences between AMI and SHAM groups at 7 d. The gut barrier impairment was also the most significant at 7 d post-AMI. We further identified the differences of microorganisms between AMI and SHAM group at 7 d. The abundance of Synergistetes phylum, Spirochaetes phylum, Lachnospiraceae family, Syntrophomonadaceae family and Tissierella Soehngenia genus was higher in AMI group compared with SHAM group at 7 d post-operation (q<0.05). Our study showed the changes of gut microbiota at day 7 post AMI which was paralleled with intestinal barrier impairment. We also identified the microbial organisms that contribute most.
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影响因子：
7.7
作者：
Cani, Patrice D.;Amar, Jacques;Burcelin, Remy
通讯作者：
Burcelin, Remy
影响因子：
2.4
作者：
Bischoff SC;Barbara G;Buurman W;Ockhuizen T;Schulzke JD;Serino M;Tilg H;Watson A;Wells JM
通讯作者：
Wells JM
影响因子：
2.7
作者：
Allen-Blevins CR;You X;Hinde K;Sela DA
通讯作者：
Sela DA
影响因子：
6.5
作者：
Clemente-Postigo, M.;Queipo-Ortuno, M. I.;Tinahones, F. J.
通讯作者：
Tinahones, F. J.
DOI：
10.1073/pnas.1000080107
发表时间：
2011-03-15
影响因子：
11.1
作者：
Caporaso, J. Gregory;Lauber, Christian L.;Knight, Rob
通讯作者：
Knight, Rob