Effect of CCR7, CXCR4 and VEGF-C on the lymph node metastasis of human pancreatic ductal adenocarcinoma.
Effect of CCR7, CXCR4 and VEGF-C on the lymph node metastasis of human pancreatic ductal adenocarcinoma.
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CCR7、CXCR4和VEGF-C对人胰腺导管腺癌淋巴结转移的影响
DOI：
10.3892/ol.2013.1261
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发表时间：
2013-05
期刊：
影响因子：
2.9
通讯作者：
Zhang S
中科院分区：
文献类型：
--
作者：
Guo J;Lou W;Ji Y;Zhang S
本研究旨在探讨趋化因子受体CCR7、CXCR4和血管内皮生长因子-C(VEGF-C)的表达与胰腺导管癌(PDAC)淋巴结转移的关系。采用实时定量逆转录聚合酶链式反应(RT-PCR)检测24例癌组织中CCR7、CXCR4和VEGF-C的转录水平，免疫组织化学法检测65例癌组织中CCR7、CXCR4和VEGF-C的蛋白表达水平。应用病理图像处理专业软件Image Pro Plus 6.0对免疫组织化学染色结果进行定量分析。癌组织中CCR7、CXCR4和VEGF-C的表达水平均显著高于癌旁正常组织。在有淋巴结转移和国际抗癌联合会(UICC)晚期的肿瘤患者中，CCR7和VEGF-C的mRNA和蛋白表达水平显著升高(P<；0.05)。转移淋巴结数目越多，CCR7的表达水平越高(P<；0.05)。CCR7和VEGF-C的mRNA和蛋白表达水平呈显著正相关(P<；0.05)。CXCR4的mRNA和蛋白表达水平与PDAC的淋巴结转移无关(P>；0.05)，而CCR7和VEGF-C的强阳性表达与PDAC的淋巴结转移显著相关(P<0.05)。
The aim of the present study was to investigate the association between the expression of chemokine receptors CCR7 and CXCR4 and vascular endothelial growth factor (VEGF)-C and the lymph node metastasis of pancreatic ductal adenocarcinoma (PDAC). The mRNA transcription levels of CCR7, CXCR4 and VEGF-C were measured in 24 specimens by real-time reverse transcription (RT)-PCR, while the protein expression levels were measured in 65 specimens by immuohistochemistry. Professional software for pathological image manipulation (Image Pro Plus 6.0) was used to quantitate the results of the immunohistochemical staining. The mRNA and protein expression levels of CCR7, CXCR4 and VEGF-C were all significantly higher in the cancer samples compared with those in the adjacent normal tissue. The CCR7 and VEGF-C mRNA and protein expression levels were significantly higher in the patients with cancer types exhibiting lymph node metastasis and an advanced International Union Against Cancer (UICC) stage (P<0.05). The greater the number of metastatic lymph nodes, the higher the levels of CCR7 expression (P<0.05). There was a significant positive linear correlation between the mRNA and protein expression levels of CCR7 and VEGF-C (P<0.05). The mRNA and protein expression levels of CXCR4 were not correlated with the lymph node metastasis (P>0.05), however the strong positive expression of CCR7 and VEGF-C was significantly associated with the lymph node metastasis of PDAC.
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DOI：
10.1016/j.jamcollsurg.2007.06.332
发表时间：
2007-10-01
影响因子：
5.2
作者：
Buechler, Markus W.;Kleeff, Joerg;Friess, Helmut
通讯作者：
Friess, Helmut
影响因子：
7.3
作者：
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通讯作者：
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影响因子：
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作者：
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5.2
作者：
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影响因子：
15.3
作者：
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通讯作者：
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