A reversed-phase capillary ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) method for comprehensive top-down/bottom-up lipid profiling.
A reversed-phase capillary ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) method for comprehensive top-down/bottom-up lipid profiling.
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DOI：
10.1007/s00216-012-5773-5
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发表时间：
2012-03
影响因子：
4.3
通讯作者：
Metz, Thomas O.
中科院分区：
文献类型：
--
作者：
Gao, Xiaoli;Zhang, Qibin;Meng, Da;Isaac, Giorgis;Zhao, Rui;Fillmore, Thomas L.;Chu, Rosey K.;Zhou, Jianying;Tang, Keqi;Hu, Zeping;Moore, Ronald J.;Smith, Richard D.;Katze, Michael G.;Metz, Thomas O.
关键词：
脂质组学是代谢组学的重要组成部分，旨在研究生命系统中的所有脂质。我们在这里提出了一个全面的自上而下/自下而上的脂质分析灵敏的毛细管UPLC-MS方法的发展和评价。使用代表脂质组的脂质标准品混合物，评价了三种不同固定相的峰容量、线性、重现性和定量限（LOQ）。当使用优化的方法时，脂质标准品的保留时间和峰丰度的相对标准偏差分别为0.29%和7.7%。在3个数量级上，线性在>0.99时是可接受的，并且LOQ低于fmol。为了证明该方法在复杂样品分析中的性能，我们分析了从人细胞系、大鼠血浆和人体皮肤组织模型中提取的脂质，分别鉴定了446、444和370种独特的脂质。总体而言，与基于色谱法与MS联用的其他全局非靶向脂质分析方法相比，该方法提供了更高的脂质组覆盖率、更高的测量灵敏度或两者兼而有之。
Lipidomics is a critical part of metabolomics and aims to study all the lipids within a living system. We present here the development and evaluation of a sensitive capillary UPLC-MS method for comprehensive top-down/bottom-up lipid profiling. Three different stationary phases were evaluated in terms of peak capacity, linearity, reproducibility, and limit of quantification (LOQ) using a mixture of lipid standards representative of the lipidome. The relative standard deviations of the retention times and peak abundances of the lipid standards were 0.29% and 7.7%, respectively, when using the optimized method. The linearity was acceptable at >0.99 over 3 orders of magnitude, and the LOQs were sub-fmol. To demonstrate the performance of the method in the analysis of complex samples, we analyzed lipids extracted from a human cell line, rat plasma, and a model human skin tissue, identifying 446, 444, and 370 unique lipids, respectively. Overall, the method provided either higher coverage of the lipidome, greater measurement sensitivity, or both, when compared to other approaches of global, untargeted lipid profiling based on chromatography coupled with MS.
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影响因子：
4.1
作者：
Ahn, Eun Jeong;Kim, Hanna;Moon, Myeong Hee
通讯作者：
Moon, Myeong Hee
DOI：
10.1073/pnas.0610634104
发表时间：
2007-03-20
影响因子：
11.1
作者：
Jain, Madhulika;Petzold, Christopher J.;Cox, Jeffery S.
通讯作者：
Cox, Jeffery S.
DOI：
10.1016/j.bbalip.2010.01.010
发表时间：
2010-08
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Han X
通讯作者：
Han X
影响因子：
16.6
作者：
Harkewicz R;Dennis EA
通讯作者：
Dennis EA
影响因子：
6.5
作者：
Fahy, Eoin;Subramaniam, Shankar;Dennis, Edward A.
通讯作者：
Dennis, Edward A.