Applications of mass spectrometry to lipids and membranes.-猫眼课题宝

DOI：

10.1146/annurev-biochem-060409-092612

发表时间：

2011

期刊：

Annual review of biochemistry

影响因子：

16.6

通讯作者：

中科院分区：

生物学1区

文献类型：

作者：

Harkewicz R;Dennis EA

来源链接：

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3410560/?report=classic

脂质组学是代谢组学的一个重要组成部分，它对生命系统中脂质（脂质组）的结构和功能进行详细的空间和时间分析和全面表征。与蛋白质组学一样，质谱法在脂质组学中也发挥着重要的分析作用，并且随着技术的发展，这一作用将继续增长。目前，存在两种基于质谱的脂质组学方法，一种是基于在分析之前将脂质划分为类别和类别，即“通过分离简化的综合脂质组学分析”（CLASS），另一种是在没有事先分离的情况下一起分析所有脂质物质，即鸟枪法。在探索各种生命系统的脂质体时，新的脂质被发现，质谱法有助于表征它们的化学结构。氘交换质谱（DXMS）被用于研究脂质和膜与蛋白质和酶的结合，成像质谱（IMS）被应用于组织中脂质的原位分析。

Lipidomics, a major part of metabolomics, constitutes the detailed analysis and global characterization, both spatial and temporal, of the structure and function of lipids (the lipidome) within a living system. As with proteomics, mass spectrometry has earned a central analytical role in lipidomics, and this role will continue to grow with technological developments. Currently, there exist two mass spectrometry-based lipidomics approaches, one based on a division of lipids into categories and classes prior to analysis, the “comprehensive lipidomics analysis by separation simplification” (CLASS), and the other in which all lipid species are analyzed together without prior separation, shotgun. In exploring the lipidome of various living systems, novel lipids are being discovered, and mass spectrometry is helping characterize their chemical structure. Deuterium exchange mass spectrometry (DXMS) is being used to investigate the association of lipids and membranes with proteins and enzymes, and imaging mass spectrometry (IMS) is being applied to the in situ analysis of lipids in tissues.