Antioxidant ability and mechanism of rhizoma Atractylodes macrocephala.
Antioxidant ability and mechanism of rhizoma Atractylodes macrocephala.
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白术的抗氧化能力及机制
DOI：
10.3390/molecules171113457
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发表时间：
2012-11-13
期刊：
影响因子：
--
通讯作者：
Chen D
中科院分区：
文献类型：
--
作者：
Li X;Lin J;Han W;Mai W;Wang L;Li Q;Lin M;Bai M;Zhang L;Chen D
白术 (AM) 在中医 (TCM) 中的应用已有约 2,000 年的历史。在这项研究中，我们首先通过·OH-清除、·O2−-清除、Fe2+-螯合、Cu2+-螯合、DPPH·-清除和ABTS+·-清除测定法测定了五种AM提取物的抗氧化水平。在测量了五种 AM 提取物的化学含量后，我们定量分析了抗氧化剂水平与化学含量之间的相关性。结果发现，总酚和总黄酮与抗氧化剂水平呈显着正相关（R分别为0.685和0.479）。相反，总糖和总皂苷与抗氧化剂水平的相关性较低（R分别为-0.272和0.244）。这意味着AM的抗氧化活性应归因于总酚类物质（包括酚酸和黄酮类化合物），而不是总糖和总皂苷。进一步分析表明，酚酸在自由基清除测定中表现出较高的 R 值（R = 0.32–1.00），而类黄酮在金属螯合测定中表现出较高的 R 值（R = 0.86 和 0.90）。综上所述，AM通过金属螯合和通过提供氢原子和提供电子来清除自由基来发挥其抗氧化作用。其金属螯合作用可能来自类黄酮，而其自由基清除作用则归因于酚酸，尤其是咖啡酸、阿魏酸和原儿茶酸。
Rhizoma Atractylodes macrocephala (AM) has been used in Traditional Chinese Medicine (TCM) for about 2,000 years. In the study, we firstly determined the antioxidant levels of five AM extracts by •OH-scavenging, •O2−-scavenging, Fe2+-chelating, Cu2+-chelating, DPPH·-scavenging, and ABTS+·-scavenging assays. After measurement of the chemical contents in five AM extracts, we quantitatively analyzed the correlations between antioxidant levels and chemical contents. It was observed that total phenolics and total flavonoids had significant positive correlations with antioxidant levels (R = 0.685 and 0.479, respectively). In contrast, total sugars and total saponins presented lower correlations with antioxidant levels (R = −0.272 and 0.244, respectively). It means that antioxidant activity of AM should be attributed to total phenolics (including phenolic acids and flavonoids), and not total sugars and total saponins. Further analysis indicated that phenolic acids exhibited higher R values with radical-scavenging assays (R = 0.32–1.00), while flavonoids showed higher R values with metal-chelating assays (R= 0.86 and 0.90). In conclusion, AM exerts its antioxidant effect through metal-chelating, and radical-scavenging which is via donating hydrogen atom and donating electron. Its metal-chelating may result from flavonoids, while its radical-scavenging can be attributed to phenolic acids, especially caffeic acid, ferulic acid, and protocatechuic acid.
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影响因子：
2.2
作者：
Li, Xican;Chen, Dongfeng;Wang, Xiaozhen
通讯作者：
Wang, Xiaozhen
影响因子：
4.1
作者：
CHEESEMAN, KH;BEAVIS, A;ESTERBAUER, H
通讯作者：
ESTERBAUER, H
DOI：
10.1073/pnas.93.24.13635
发表时间：
1996-11-26
影响因子：
11.1
作者：
Keyer, K;Imlay, JA
通讯作者：
Imlay, JA
DOI：
10.1016/j.ifset.2005.09.001
发表时间：
2006-06-01
影响因子：
6.6
作者：
Tsimogiannis, Dimitrios I.;Oreopoulou, Vassiliki
通讯作者：
Oreopoulou, Vassiliki
DOI：
10.1073/pnas.1202367109
发表时间：
2012-06-26
影响因子：
11.1
作者：
Hashizume, Osamu;Shimizu, Akinori;Hayashi, Jun-Ichi
通讯作者：
Hayashi, Jun-Ichi