OsRAD51C is essential for double-strand break repair in rice meiosis.
OsRAD51C is essential for double-strand break repair in rice meiosis.
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OsRAD51C 对于水稻减数分裂中的双链断裂修复至关重要。
DOI：
10.3389/fpls.2014.00167
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发表时间：
2014
影响因子：
5.6
通讯作者：
Cheng Z
中科院分区：
文献类型：
--
作者：
Tang D;Miao C;Li Y;Wang H;Liu X;Yu H;Cheng Z
RAD51C是RAD51的旁系同源物之一，其在通过同源重组进行DNA双链断裂修复中起重要作用。在这里，我们鉴定并表征了OsRAD51C，人RAD51C的水稻同源物。Osrad51c突变体植物的营养生长正常，但表现出完全的雄性和雌性不育。细胞学检查显示同源配对和突触严重破坏。Osrad51c减数分裂中期I发生大量染色体断裂，并被CRC1突变完全抑制。免疫荧光分析表明，OsRAD51C定位于细线期至粗线期早期的染色体上，并且OsRAD51C的正常加载依赖于OsREC 8、PAIR 2和PAIR 3。此外，ZEP 1没有正确定位在OsRAD 51C中，表明OsRAD 51C是联会复合体组装所必需的。我们的研究还提供了证据，支持在生物体之间的功能分化的RAD51C。
RAD51C is one of the RAD51 paralogs that plays an important role in DNA double-strand break repair by homologous recombination. Here, we identified and characterized OsRAD51C, the rice homolog of human RAD51C. The Osrad51c mutant plant is normal in vegetative growth but exhibits complete male and female sterility. Cytological investigation revealed that homologous pairing and synapsis were severely disrupted. Massive chromosome fragmentation occurred during metaphase I in Osrad51c meiocytes, and was fully suppressed by the CRC1 mutation. Immunofluorescence analysis showed that OsRAD51C localized onto the chromosomes from leptotene to early pachytene during prophase I, and that normal loading of OsRAD51C was dependent on OsREC8, PAIR2, and PAIR3. Additionally, ZEP1 did not localize properly in Osrad51c, indicating that OsRAD51C is required for synaptonemal complex assembly. Our study also provided evidence in support of a functional divergence in RAD51C among organisms.
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DOI：
10.1083/jcb.200811079
发表时间：
2009-05-18
期刊：
The Journal of cell biology
影响因子：
--
作者：
Badie S;Liao C;Thanasoula M;Barber P;Hill MA;Tarsounas M
通讯作者：
Tarsounas M
影响因子：
14.9
作者：
BIRKENBIHL, RP;SUBRAMANI, S
通讯作者：
SUBRAMANI, S
影响因子：
4.8
作者：
French, CA;Masson, JY;Thacker, J
通讯作者：
Thacker, J
影响因子：
4.4
作者：
Albala, JS;Thelen, MP;Lennon, GG
通讯作者：
Lennon, GG
影响因子：
6.9
作者：
Kou, Yanjun;Chang, Yuxiao;Wang, Shiping
通讯作者：
Wang, Shiping