Channel density and porosity of degradable bridging scaffolds on axon growth after spinal injury.
Channel density and porosity of degradable bridging scaffolds on axon growth after spinal injury.
复制标题
脊柱损伤后轴突生长的可降解支架的通道密度和孔隙率。
DOI：
10.1016/j.biomaterials.2012.12.002
复制
发表时间：
2013-03
期刊：
影响因子：
14
通讯作者：
Shea, Lonnie D.
中科院分区：
文献类型：
--
作者：
Thomas, Aline M.;Kubilius, Matthew B.;Holland, Samantha J.;Seidlits, Stephanie K.;Boehler, Ryan M.;Anderson, Aileen J.;Cummings, Brian J.;Shea, Lonnie D.
植入受伤脊髓的桥可以稳定损伤，同时也支持和指导轴突生长。桥的结构对其功能至关重要，其孔隙支持细胞渗透，将植入物与宿主整合在一起，并有通道引导轴突伸长。在这里，我们开发了一种蔗糖纤维模板来创建聚（丙交酯-共-乙交酯）多通道桥，用于植入侧半部分，其通道数量相对于之前的桥增加了3倍，总体孔隙率范围约为70%至90%。植入大鼠和小鼠模型后，在所有条件下在通道内观察轴突。与之前通道较少的桥相比，桥内的轴突密度增加了近 7 倍。此外，增加桥孔隙率大大增加了轴突的数量，这与整个桥的细胞浸润程度相关。对这些细胞类型的分析发现，在较高孔隙率的桥内，成熟少突胶质细胞的存在增加。这些结果表明，通道和桥孔隙度会影响损伤后轴突的重新生长。这些桥梁提供了一种平台技术，能够与再生因子的输送相结合，以实现功能恢复的最终目标。
Bridges implanted into the injured spinal cord function to stabilize the injury, while also supporting and directing axon growth. The architecture of the bridge is critical to its function, with pores to support cell infiltration that integrates the implant with the host and channels to direct axon elongation. Here, we developed a sucrose fiber template to create poly(lactide-co-glycolide) multiple channel bridges for implantation into a lateral hemisection that had a 3-fold increase in channel number relative to previous bridges and an overall porosity ranging from approximately 70% to 90%. Following implantation into rat and mouse models, axons were observed within channels for all conditions. The axon density within the bridge increased nearly 7-fold relative to previous bridges with fewer channels. Furthermore, increasing the bridge porosity substantially increased the number of axons, which correlated with the extent of cell infiltration throughout the bridge. Analysis of these cell types identified an increased presence of mature oligodendrocytes within the bridge at higher porosities. These results demonstrate that channels and bridge porosity influence the re-growth of axons through the injury. These bridges provide a platform technology capable of being combined with the delivery of regenerative factors for the ultimate goal of achieving functional recovery.
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DOI：
10.1523/jneurosci.5524-09.2011
发表时间：
2011-04-20
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Wang Y;Cheng X;He Q;Zheng Y;Kim DH;Whittemore SR;Cao QL
通讯作者：
Cao QL
影响因子：
6.1
作者：
Dougherty, KD;Dreyfus, CF;Black, IB
通讯作者：
Black, IB
影响因子：
5.3
作者：
Amankulor, Nduka M.;Hambardzumyan, Dolores;Holland, Eric C.
通讯作者：
Holland, Eric C.
DOI：
10.1073/pnas.052678899
发表时间：
2002-03-05
影响因子：
11.1
作者：
Teng, YD;Lavik, EB;Snyder, EY
通讯作者：
Snyder, EY
影响因子：
14
作者：
Yu, TT;Shoichet, MS
通讯作者：
Shoichet, MS