Magnetic core-shell nanoparticles for drug delivery by nebulization.
Magnetic core-shell nanoparticles for drug delivery by nebulization.
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DOI：
10.1186/1477-3155-11-1
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发表时间：
2013-01-23
影响因子：
10.2
通讯作者：
Gun'ko YK
中科院分区：
文献类型：
--
作者：
Verma NK;Crosbie-Staunton K;Satti A;Gallagher S;Ryan KB;Doody T;McAtamney C;MacLoughlin R;Galvin P;Burke CS;Volkov Y;Gun'ko YK
雾化疗法在有效治疗包括肺癌在内的各种疾病方面具有巨大的潜力。在此背景下，迫切需要开发适合雾化给药的新型纳米载体。为了满足这一需求，我们在Fe3O4磁性纳米颗粒（MNPs）表面涂覆聚合物聚乳酸-羟基乙酸（PLGA）后，合成并表征了一种生物相容性药物递送载体。这些工程纳米颗粒的聚合物外壳装载了一种潜在的抗癌药物槲皮素，并评估了它们通过雾化靶向肺癌细胞的适用性。电镜测得发育的MNPs和PLGA-MNPs的平均粒径为9.6 nm和53.2 nm，动态光散射测得其水动力膨胀度分别为54.3 nm和293.4 nm。利用一系列标准化的生物学测试，结合基于细胞的自动图像采集和分析程序，结合实时阻抗传感，我们证实了开发的基于mnp的纳米载体系统具有生物相容性，当高达100 μg/ml的PLGA-MNP应用于培养的人肺上皮细胞时，没有观察到细胞毒性。此外，通过谷胱甘肽和IL-6分泌测定，在治疗后1、4和7天，经鼻给药的PLGA-MNP制剂在小鼠体内耐受良好。为了模拟药物进入肺部的气溶胶形成，我们将槲皮素负载的PLGA-MNPs应用于人肺癌细胞系A549，然后进行一轮雾化。载药的PLGA-MNPs显著减少了A549细胞的存活数量，这与雾化或直接移液的效果相当。我们开发了一种基于磁性核壳纳米粒子的纳米载体系统，并评估了其通过气溶胶给药的给药能力的可行性。这项研究对通过吸入途径靶向递送治疗药物和难溶性药物化合物具有指导意义。
Aerosolized therapeutics hold great potential for effective treatment of various diseases including lung cancer. In this context, there is an urgent need to develop novel nanocarriers suitable for drug delivery by nebulization. To address this need, we synthesized and characterized a biocompatible drug delivery vehicle following surface coating of Fe3O4 magnetic nanoparticles (MNPs) with a polymer poly(lactic-co-glycolic acid) (PLGA). The polymeric shell of these engineered nanoparticles was loaded with a potential anti-cancer drug quercetin and their suitability for targeting lung cancer cells via nebulization was evaluated. Average particle size of the developed MNPs and PLGA-MNPs as measured by electron microscopy was 9.6 and 53.2 nm, whereas their hydrodynamic swelling as determined using dynamic light scattering was 54.3 nm and 293.4 nm respectively. Utilizing a series of standardized biological tests incorporating a cell-based automated image acquisition and analysis procedure in combination with real-time impedance sensing, we confirmed that the developed MNP-based nanocarrier system was biocompatible, as no cytotoxicity was observed when up to 100 μg/ml PLGA-MNP was applied to the cultured human lung epithelial cells. Moreover, the PLGA-MNP preparation was well-tolerated in vivo in mice when applied intranasally as measured by glutathione and IL-6 secretion assays after 1, 4, or 7 days post-treatment. To imitate aerosol formation for drug delivery to the lungs, we applied quercitin loaded PLGA-MNPs to the human lung carcinoma cell line A549 following a single round of nebulization. The drug-loaded PLGA-MNPs significantly reduced the number of viable A549 cells, which was comparable when applied either by nebulization or by direct pipetting. We have developed a magnetic core-shell nanoparticle-based nanocarrier system and evaluated the feasibility of its drug delivery capability via aerosol administration. This study has implications for targeted delivery of therapeutics and poorly soluble medicinal compounds via inhalation route.
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影响因子：
8
作者：
Akbarzadeh A;Mikaeili H;Zarghami N;Mohammad R;Barkhordari A;Davaran S
通讯作者：
Davaran S
影响因子：
3.5
作者：
GrassiSchultheiss, PP;Heller, F;Dobson, J
通讯作者：
Dobson, J
影响因子：
2.9
作者：
Davies, Gemma-Louise;Corr, Serena A.;Gun'ko, Yurii K.
通讯作者：
Gun'ko, Yurii K.
影响因子：
38.3
作者：
Dames, Petra;Gleich, Bernhard;Rudolph, Carsten
通讯作者：
Rudolph, Carsten
影响因子：
--
作者：
Gallagher, Joseph J.;Tekoriute, Renata;Lynch, Marina
通讯作者：
Lynch, Marina