RTPrimerDB: the portal for real-time PCR primers and probes.
RTPrimerDB: the portal for real-time PCR primers and probes.
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RTPRIMERDB：实时PCR引物和探针的门户。
DOI：
10.1093/nar/gkn777
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发表时间：
2009-01
影响因子：
14.9
通讯作者：
Pattyn F
中科院分区：
文献类型：
--
作者：
Lefever S;Vandesompele J;Speleman F;Pattyn F
RTPrimerDB(http://www.rtprimerdb.org)是一个可免费访问的数据库和分析工具，用于实时定量聚合酶链式反应分析。RTPrimerDB包括带有用户提交的检测的记录，这些检测链接到参考数据库中的基因组信息，并使用计算机内检测评估系统进行质量控制。旨在评估特异性和检测可能对扩增效率产生负面影响的特征的引物评估工具被组合到一条管道中，以测试定制设计的引物和探针序列。改进的用户反馈系统引导用户和提交者输入关于实验评估分析的实际评论和细节。该数据库与参考数据库相连，以便提交在Entrez gene和RefSeq中正式注册的所有基因和生物的分析。RTPrimerDB中的记录被分配唯一且稳定的标识符。这些内容是通过基于网络的交互式搜索系统提供的，可以下载最近开发的RDML格式和批量导出文件。RTPrimerDB是一个一站式门户，提供高质量和高度注释的实时PCR分析。
RTPrimerDB (http://www.rtprimerdb.org) is a freely accessible database and analysis tool for real-time quantitative PCR assays. RTPrimerDB includes records with user submitted assays that are linked to genome information from reference databases and quality controlled using an in silico assay evaluation system. The primer evaluation tools intended to assess the specificity and to detect features that could negatively affect the amplification efficiency are combined into a pipeline to test custom-designed primer and probe sequences. An improved user feedback system guides users and submitters to enter practical remarks and details about experimental evaluation analyses. The database is linked with reference databases to allow the submission of assays for all genes and organisms officially registered in Entrez Gene and RefSeq. Records in RTPrimerDB are assigned unique and stable identifiers. The content is provided via an interactive web-based search system and is available for download in the recently developed RDML format and as bulk export file. RTPrimerDB is a one-stop portal for high-quality and highly annotated real-time PCR assays.
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影响因子：
14.9
作者：
Flicek P;Aken BL;Beal K;Ballester B;Caccamo M;Chen Y;Clarke L;Coates G;Cunningham F;Cutts T;Down T;Dyer SC;Eyre T;Fitzgerald S;Fernandez-Banet J;Gräf S;Haider S;Hammond M;Holland R;Howe KL;Howe K;Johnson N;Jenkinson A;Kähäri A;Keefe D;Kokocinski F;Kulesha E;Lawson D;Longden I;Megy K;Meidl P;Overduin B;Parker A;Pritchard B;Prlic A;Rice S;Rios D;Schuster M;Sealy I;Slater G;Smedley D;Spudich G;Trevanion S;Vilella AJ;Vogel J;White S;Wood M;Birney E;Cox T;Curwen V;Durbin R;Fernandez-Suarez XM;Herrero J;Hubbard TJ;Kasprzyk A;Proctor G;Smith J;Ureta-Vidal A;Searle S
通讯作者：
Searle S
影响因子：
46.9
作者：
Taylor, Chris F.;Field, Dawn;Sansone, Susanna-Assunta;Aerts, Jan;Apweiler, Rolf;Ashburner, Michael;Ball, Catherine A.;Binz, Pierre-Alain;Bogue, Molly;Booth, Tim;Brazma, Alvis;Brinkman, Ryan R.;Clark, Adam Michael;Deutsch, Eric W.;Fiehn, Oliver;Fostel, Jennifer;Ghazal, Peter;Gibson, Frank;Gray, Tanya;Grimes, Graeme;Hancock, John M.;Hardy, Nigel W.;Hermjakob, Henning;Julian, Randall K., Jr.;Kane, Matthew;Kettner, Carsten;Kinsinger, Christopher;Kolker, Eugene;Kuiper, Martin;Le Novere, Nicolas;Leebens-Mack, Jim;Lewis, Suzanna E.;Lord, Phillip;Mallon, Ann-Marie;Marthandan, Nishanth;Masuya, Hiroshi;McNally, Ruth;Mehrle, Alexander;Morrison, Norman;Orchard, Sandra;Quackenbush, John;Reecy, James M.;Robertson, Donald G.;Rocca-Serra, Philippe;Rodriguez, Henry;Rosenfelder, Heiko;Santoyo-Lopez, Javier;Scheuermann, Richard H.;Schober, Daniel;Smith, Barry;Snape, Jason;Stoeckert, Christian J., Jr.;Tipton, Keith;Sterk, Peter;Untergasser, Andreas;Vandesompele, Jo;Wiemann, Stefan
通讯作者：
Wiemann, Stefan
影响因子：
14.9
作者：
Maglott, Donna;Ostell, Jim;Pruitt, Kim D;Tatusova, Tatiana
通讯作者：
Tatusova, Tatiana
影响因子：
14.9
作者：
Pruitt KD;Tatusova T;Maglott DR
通讯作者：
Maglott DR
影响因子：
14.9
作者：
Karolchik, D.;Kuhn, R. M.;Baertsch, R.;Barber, G. P.;Clawson, H.;Diekhans, M.;Giardine, B.;Harte, R. A.;Hinrichs, A. S.;Hsu, F.;Kober, K. M.;Miller, W.;Pedersen, J. S.;Pohl, A.;Raney, B. J.;Rhead, B.;Rosenbloom, K. R.;Smith, K. E.;Stanke, M.;Thakkapallayil, A.;Trumbower, H.;Wang, T.;Zweig, A. S.;Haussler, D.;Kent, W. J.
通讯作者：
Kent, W. J.