A Polysaccharide From the Whole Plant of Plantago asiatica L. Enhances the Antitumor Activity of Dendritic Cell-Based Immunotherapy Against Breast Cancer.
A Polysaccharide From the Whole Plant of Plantago asiatica L. Enhances the Antitumor Activity of Dendritic Cell-Based Immunotherapy Against Breast Cancer.
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DOI：
10.3389/fphar.2021.678865
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发表时间：
2021
影响因子：
5.6
通讯作者：
Lin J
中科院分区：
文献类型：
--
作者：
Gao J;Zhang YN;Cui J;Zhang J;Ming Y;Hao Z;Xu H;Cheng N;Zhang D;Jin Y;Lin D;Lin J
树突状细胞 (DC) 是介导 T 细胞免疫反应的最有效的专业抗原呈递细胞 (APC)。乳腺癌是最常见的诊断疾病之一，其死亡率高于人类和犬科动物的任何其他癌症。车前草多糖（PLP）是从车前草全草中提取的，具有促进DCs成熟的作用。在这项研究中，我们发现PLP可以在体外和体内上调DC的成熟。 PLP 激活的 DC 可以刺激淋巴细胞增殖并将幼稚 T 细胞分化为细胞毒性 T 细胞。 PLP和CIPp犬乳腺肿瘤细胞裂解物脉冲的DC增强了肿瘤抗原特异性淋巴细胞反应，并且PLP和CIPp细胞裂解物联合刺激的DC与对CIPp细胞具有很大细胞毒性的淋巴细胞共培养。在4T1小鼠乳腺肿瘤模型中，PLP可以通过在肿瘤微环境中招募DC、巨噬细胞以及CD4+和CD8+T细胞来控制乳腺肿瘤的大小并提高免疫力。这些结果表明PLP可以达到免疫治疗效果并提高乳腺肿瘤模型的免疫力。
Dendritic cells (DCs) are the most potent professional antigen-presenting cells (APCs) that mediate T-cell immune responses. Breast cancer is one of the most commonly diagnosed diseases and its mortality rate is higher than any other cancer in both humans and canines. Plantain polysaccharide (PLP), extracted from the whole plant of Plantago asiatica L., could promote the maturation of DCs. In this research, we found that PLP could upregulate the maturation of DCs both in vitro and in vivo. PLP-activated DCs could stimulate lymphocytes’ proliferation and differentiate naive T cells into cytotoxic T cells. Tumor antigen-specific lymphocyte responses were enhanced by PLP and CIPp canine breast tumor cells lysate-pulsed DCs, and PLP and CIPp-cell-lysate jointly stimulated DCs cocultured with lymphocytes having the great cytotoxicity on CIPp cells. In the 4T1 murine breast tumor model, PLP could control the size of breast tumors and improve immunity by recruiting DCs, macrophages, and CD4+ and CD8+ T cells in the tumor microenvironment. These results indicated that PLP could achieve immunotherapeutic effects and improve immunity in the breast tumor model.
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DOI：
10.1038/nrc3258
发表时间：
2012-03-22
期刊：
Nature reviews. Cancer
影响因子：
--
作者：
通讯作者：
--
DOI：
10.4049/jimmunol.0803484
发表时间：
2009-03-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Xiao Z;Casey KA;Jameson SC;Curtsinger JM;Mescher MF
通讯作者：
Mescher MF
来自车前子种子的新型多糖通过 Toll 样受体 4 诱导树突状细胞成熟
影响因子：
5.6
作者：
Huang, Danfei;Nie, Shaoping;Xie, Mingyong
通讯作者：
Xie, Mingyong
影响因子：
15.9
作者：
Carreno, Beatriz M.;Becker-Hapak, Michelle;Linette, Gerald P.
通讯作者：
Linette, Gerald P.
犬乳腺肿瘤的转录组特征及其与人乳腺癌的比较。
影响因子：
5.2
作者：
Lee KH;Park HM;Son KH;Shin TJ;Cho JY
通讯作者：
Cho JY