A competitive transcription factor binding mechanism determines the timing of late cell cycle-dependent gene expression.
A competitive transcription factor binding mechanism determines the timing of late cell cycle-dependent gene expression.
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DOI：
10.1016/j.molcel.2010.02.030
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发表时间：
2010-04-09
期刊：
影响因子：
16
通讯作者：
Sharrocks AD
中科院分区：
文献类型：
--
作者：
Darieva Z;Clancy A;Bulmer R;Williams E;Pic-Taylor A;Morgan BA;Sharrocks AD
转录控制是由激活蛋白和阻遏蛋白的拮抗活性来实现的。在酿酒酵母中，含有MADS盒蛋白Mcm 1 p的转录因子复合物是G2/M和M/G1转换时细胞周期依赖性转录的关键调节因子。同源域阻遏蛋白Yox 1 p与Mcm 1 p形成复合物，控制基因表达的时间。在这里，我们表明，Yox 1 p相互作用与Mcm 1 p通过一个基序位于N末端的同源结构域。Yox 1 p作为一个转录抑制因子，通过与叉头转录激活蛋白Fkh 2 p竞争，通过蛋白质-蛋白质相互作用在Mcm 1 p调控基因的一个子集的启动子结合Mcm 1 p。重要的是，这种竞争不是通过结合通常观察到的相同DNA位点。因此，本研究描述了一种不同的机制，用于确定细胞周期依赖性基因表达的时间，涉及阻遏物和激活蛋白中的短肽基序之间的竞争，以与共同的结合伴侣相互作用。阻遏蛋白Yox 1 p使用一个短的疏水基序与Mcm 1 p结合Yox 1 p和叉头转录激活因子Fkh 2 p竞争性地与Mcm 1 p结合Yox 1 p和Fkh 2 p的时间结合决定了细胞周期转录的时间
Transcriptional control is exerted by the antagonistic activities of activator and repressor proteins. In Saccharomyces cerevisiae, transcription factor complexes containing the MADS box protein Mcm1p are key regulators of cell cycle-dependent transcription at both the G2/M and M/G1 transitions. The homeodomain repressor protein Yox1p acts in a complex with Mcm1p to control the timing of gene expression. Here, we show that Yox1p interacts with Mcm1p through a motif located N terminally to its homeodomain. Yox1p functions as a transcriptional repressor by competing with the forkhead transcription activator protein Fkh2p for binding to Mcm1p through protein-protein interactions at promoters of a subset of Mcm1p-regulated genes. Importantly, this competition is not through binding the same DNA site that is commonly observed. Thus, this study describes a different mechanism for determining the timing of cell cycle-dependent gene expression that involves competition between short peptide motifs in repressor and activator proteins for interaction with a common binding partner. ► The repressor protein Yox1p uses a short hydrophobic motif to bind to Mcm1p ► Yox1p and the forkhead transcriptional activator, Fkh2p, competitively bind to Mcm1p ► Competition is driven through mutually exclusive protein-protein interactions ► Temporal binding of Yox1p and Fkh2p determines the timing of cell-cycle transcription
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影响因子：
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作者：
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