Taxonogenomics: Genome of a New Organism First Genome Sequences of Buffalo Coronavirus from Water Buffaloes in Bangladesh-猫眼课题宝

分类基因组学：新生物体的基因组来自孟加拉国水牛的水牛冠状病毒的第一个基因组序列

DOI：

发表时间：

期刊：

影响因子：

通讯作者：

中科院分区：

文献类型：

作者：

S. Lau;A. K. L. Tsang;S. S. Ahmed;M. Mahbub Alam;Z. Ahmed;Po;K.;P. C. Y. Woo

We report the complete genome sequences of a buffalo coronavirus (BufCoV HKU26) detected from the faecal samples of two domestic water buffaloes (Bubalus bubalis) in Bangladesh. They possessed 98–99% nucleotide identities to bovine coronavirus (BCoV) genomes, supporting BufCoV HKU26 as a member of Betacoronavirus 1. Nevertheless, BufCoV HKU26 possessed distinct accessory proteins between spike and envelope compared to BCoV. Sugar-binding residues in the N-terminal domain of S protein in BCoV are conserved in BufCoV HKU26. Coronaviruses are classified into four genera, with bat coro-naviruses known as the gene source of Alphacoronavirus and Betacoronavirus, and avian coronaviruses as the gene source of Gammacoronavirus and Deltacoronavirus [1,2]. However, lineage A Betacoronavirus is unique among the genus in originating in rodents instead of bats [3]. Lineage A Betacoronavirus comprises several coronavirus species, including murine coronavirus, human coronavirus HKU1, Chinese Rattus coronavirus HKU24, rabbit coronavirus HKU14 and Betacoronavirus 1 [3–5]. Beta-coronavirus 1 is best known for its tendency for recombination and interspecies transmission among various mammalian species [3,6,7]. In particular, human coronavirus OC43 (HCoV OC43) likely originated from a relatively recent zoonotic transmission event, with the most recent common ancestor of HCoV OC43 and bovine coronavirus (BCoV) dating to around 1890 [8]. Besides cattle, BCoV-like viruses have been detected in various ungulates, including water buffalo calves with gastroenteritis in Italy [9,10]. However, only partial gene sequences , the longest one being ~9.6 kb spanning ORF1b to nucleocapsid (N), were obtained from the buffalo viruses [9,10]. We report the complete genome sequences of a buffalo coronavirus (BufCoV HKU26) detected from the faecal samples of two domestic adult water buffaloes (Bubalus bubalis) in Bangladesh. RT-PCR for coronavirus detection, and complete genome sequencing and analysis were performed as described previously [11,12]. The genomes of BufCoV HKU26 strains B1-24F and B1-28F were 31 021 and 30 975 in length, with G+C content of 40%. They possessed 98–99% nucleotide identities to the genomes of BCoVs, supporting the classification of BufCoV HKU26 as a member of the species Betacoronavirus 1 (Fig. 1). The genome organization is also characteristic of line-age A Betacoronavirus, with the putative transcription regulatory sequence motif 5 0-C(U/C)AAAC-3 0 (Fig. 1). The two BufCoV HKU26 genomes encode five putative accessory proteins