Blood Gene Expression and Vascular Function Biomarkers in Professional Saturation Diving.
Blood Gene Expression and Vascular Function Biomarkers in Professional Saturation Diving.
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DOI：
10.3389/fphys.2018.00937
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发表时间：
2018
影响因子：
4
通讯作者：
Eftedal I
中科院分区：
文献类型：
--
作者：
Kiboub FZ;Møllerløkken A;Hjelde A;Flatberg A;Loennechen Ø;Eftedal I
饱和潜水是在人工干预下进行水下作业的一种既定方式。在工作时，潜水员必须适应高压环境。在这项研究中，研究了全基因组的基因表达和筛选的血管功能的血浆生物标志物。我们还检查了抗氧化维生素补充剂是否会影响结果。这项研究包括20名男性专业潜水员，其中13人在持续7-14天的饱和期内每天服用1000和30毫克的维生素C和E补充剂。潜水是在氧分压(PpO2)为40kpa的氦氧气氛中进行的，潜水深度为100-115m的海水(MSW)，潜水员在此进行水下作业，最大深度为125 MSW，氧分压为60kPa.分别在饱和前和饱和后即刻采集静脉血。在基因表达谱之后，分析饱和后基因活性的变化。检测血浆中炎症、内皮功能和纤溶的蛋白生物标志物：IL-6、CRP、ICAM-1、纤维蛋白原和PAI-1。饱和后基因表达的变化表明，通过广泛下调参与氧气运输的因素，包括血红素、血红蛋白和红细胞，适应了升高的ppO2。主要的内源性抗氧化剂超氧化物歧化酶1、过氧化氢酶和谷胱甘肽合成酶上调，参与免疫活动和炎症信号通路的基因表达增加。抗氧化剂维生素补充剂对饱和后基因表达谱或血管功能生物标志物没有影响，这意味着潜水员通过内源性抗氧化剂防御维持了体内平衡。
Saturation diving is an established way to conduct subsea operations with human intervention. While working, the divers must acclimatize to the hyperbaric environments. In this study, genome-wide gene expression and selected plasma biomarkers for vascular function were investigated. We also examined whether antioxidant vitamin supplements affected the outcome. The study included 20 male professional divers, 13 of whom took vitamin C and E supplements in doses of 1,000 and 30 mg daily during saturation periods that lasted 7–14 days. The dives were done in a heliox atmosphere with 40 kPa oxygen partial pressure (ppO2) to a depth of 100–115 m of sea-water (msw), from which the divers performed in-water work excursions to a maximum depth of 125 msw with 60 kPa ppO2. Venous blood was collected immediately before and after saturation. Following gene expression profiling, post-saturation gene activity changes were analyzed. Protein biomarkers for inflammation, endothelial function, and fibrinolysis: Il-6, CRP, ICAM-1, fibrinogen, and PAI-1, were measured in plasma. Post-saturation gene expression changes indicated acclimatization to elevated ppO2 by extensive downregulation of factors involved in oxygen transport, including heme, hemoglobin, and erythrocytes. Primary endogenous antioxidants; superoxide dismutase 1, catalase, and glutathione synthetase, were upregulated, and there was increased expression of genes involved in immune activity and inflammatory signaling pathways. The antioxidant vitamin supplements had no effect on post-saturation gene expression profiles or vascular function biomarkers, implying that the divers preserved their homeostasis through endogenous antioxidant defenses.
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影响因子：
120.7
作者：
KANNEL, WB;WOLF, PA;DAGOSTINO, RB
通讯作者：
DAGOSTINO, RB
影响因子：
4.6
作者：
Eftedal, Ingrid;Ljubkovic, Marko;Dujic, Zeljko
通讯作者：
Dujic, Zeljko
影响因子：
5.8
作者：
Brubakk, Alf O.;Ross, John A. S.;Thom, Stephen R.
通讯作者：
Thom, Stephen R.
影响因子：
2.7
作者：
Perk, Joep;De Backer, Guy;Zannad, Faiez
通讯作者：
Zannad, Faiez
影响因子：
37.8
作者：
Pearson, TA;Mensah, GA;Vinicor, F
通讯作者：
Vinicor, F