Analysis of the genes of the OEE1 and OEE3 proteins of the photosystem II complex from Chlamydomonas reinhardtii-猫眼课题宝

莱茵衣藻光系统 II 复合体 OEE1 和 OEE3 蛋白基因分析

DOI：

发表时间：

1989

期刊：

Plant Molecular Biology

影响因子：

5.1

通讯作者：

中科院分区：

生物学2区

文献类型：

作者：

S. Mayfield;M. Schirmer;G. Frank;H. Zuber;J. Rochaix

莱茵衣藻放氧复合物的 33 kDa (OEE1) 和 16 kDa (OEE3) 多肽的核基因序列已确定。莱茵衣藻与高等植物和蓝藻的 OEE1 蛋白序列之间的比较显示出 67% 和 47% 的同源性。相比之下，莱茵衣藻和高等植物与 OEE3 的总体同源性仅为 28%，这主要限于蛋白质的中心部分。莱茵衣藻蛋白的转运肽由 52 个 (OEE1) 和最有可能的 51 个 (OEE1) 氨基酸组成。它们具有一个碱性氨基末端区域，并且至少在 OEE1 的情况下，在其羧基末端有一个典型的类囊体腔蛋白的疏水片段。基因组和 cDNA 克隆的比较表明，OEE1 和 OEE3 基因分别包含 5 个和 4 个内含子，其中一些位于转运肽的编码序列内。

The sequences of the nuclear genes of the 33 kDa (OEE1) and the 16 kDa (OEE3) polypeptides of the oxygen evolving complex of Chlamydomonas reinhardtii have been established. Comparison between the OEE1 protein sequences of C. reinhardtii and higher plants and cyanobacteria reveals 67 and 47% homology. In contrast, C. reinhardtii and higher plants have only 28% overall homology for OEE3 which is mostly limited to the central portion of the protein. The transit peptides of the C. reinhardtii proteins consist of 52 (OEE1) and, most likely, 51 (OEE1) amino acids. They have a basic amino terminal region and, at least in the case of OEE1, a hydrophobic segment at their carboxy terminal end typical of thylakoid lumen proteins. Comparison of the genomic and cDNA clones indicates that the OEE1 and OEE3 genes contain five and four introns, respectively, some of which are located within the coding sequences of the transit peptides.