A pan-cancer analysis of the prognostic and immunological role of β-actin (ACTB) in human cancers.
A pan-cancer analysis of the prognostic and immunological role of β-actin (ACTB) in human cancers.
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DOI：
10.1080/21655979.2021.1973220
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发表时间：
2021-12
期刊：
影响因子：
4.9
通讯作者：
Zhou Y
中科院分区：
文献类型：
--
作者：
Gu Y;Tang S;Wang Z;Cai L;Lian H;Shen Y;Zhou Y
β -肌动蛋白（ACTB）是一种高度保守的细胞骨架结构蛋白，多年来一直被认为是一种常见的管家基因，并被用作参考基因。然而，越来越多的证据表明，ACTB在多种癌症中异常表达，从而改变细胞骨架，影响肿瘤的侵袭和转移。本研究旨在探讨ACTB在泛癌中的功能及临床意义。基于基因表达综合数据库和肿瘤基因组图谱，探讨ACTB在33种肿瘤的预后和免疫调节中的作用。ACTB在肿瘤与癌旁正常组织中存在差异表达，ACTB表达与肿瘤患者预后有显著相关性。在大多数癌症中，ACTB表达与免疫细胞浸润、免疫检查点和其他免疫调节剂有关。通过CancerSEA发现ACTB与各种类型癌症的转移和侵袭之间存在相关性。此外，ACTB的作用机制还包括局灶黏附和肌动蛋白调控相关途径。实时定量聚合酶链反应验证ACTB的表达。敲低ACTB可通过NF-κB和Wnt/β-catenin途径抑制头颈部鳞癌细胞的迁移和侵袭。我们首次对ACTB进行泛肿瘤研究，深入了解了ACTB在不同肿瘤中的预后和免疫学作用，表明ACTB可能是癌症预后不良和免疫浸润的潜在生物标志物，ACTB作为癌症参考基因的作用受到了挑战。
Beta-actin (ACTB), a highly conserved cytoskeleton structural protein, has been regarded as a common housekeep gene and used as a reference gene for years. However, accumulating evidence indicates that ACTB is abnormally expressed in multiple cancers and hence changes the cytoskeleton to affect the invasiveness and metastasis of tumors. This study aimed to investigate the function and clinical significance of ACTB in pan-cancer. The role of ACTB for prognosis and immune regulation across 33 tumors was explored based on the datasets of gene expression omnibus and the cancer genome atlas. Differential expression of ACTB was found between cancer and adjacent normal tissues, and significant associations was found between ACTB expression and prognosis of tumor patients. In most cancers, ACTB expression was associated with immune cells infiltration, immune checkpoints and other immune modulators. Relevance between ACTB and metastasis and invasion was identified in various types of cancers by CancerSEA. Moreover, focal adhesion and actin regulation-associated pathways were included in the functional mechanisms of ACTB. The expression of ACTB was verified by quantitative real-time polymerase chain reaction. Knockdown of ACTB inhibited head and neck squamous carcinoma cell migration and invasion by NF-κB and Wnt/β-catenin pathways. Our first pan-cancer study of ACTB offers insight into the prognostic and immunological roles of ACTB across different tumors, indicating ACTB may be a potential biomarker for poor prognosis and immune infiltration in cancers, and the role of ACTB as a reference gene in cancers was challenged.
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影响因子：
5.2
作者：
Chen, Min-Shan;Lo, Yuan-Hung;Shroyer, Noah F.
通讯作者：
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作者：
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作者：
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通讯作者：
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