Photocrosslinkable laminin-functionalized polyethylene glycol hydrogel for intervertebral disc regeneration.
Photocrosslinkable laminin-functionalized polyethylene glycol hydrogel for intervertebral disc regeneration.
复制标题
可光叠型层粘连蛋白官能化的聚乙烯甘油水凝胶用于椎间盘再生。
DOI：
10.1016/j.actbio.2013.11.013
复制
发表时间：
2014-03
影响因子：
9.7
通讯作者：
Setton, Lori A.
中科院分区：
文献类型：
--
作者：
Francisco, Aubrey T.;Hwang, Priscilla Y.;Jeong, Claire G.;Jing, Liufang;Chen, Jun;Setton, Lori A.
Please try later.
Intervertebral disc (IVD) disorders and age-related degeneration are believed to contribute to low back pain. There is significant interest in cell-based strategies for regenerating the nucleus pulposus (NP) region of the disc; however, few scaffolds have been evaluated for their ability to promote or maintain an immature NP cell phenotype. Previous studies have shown that NP cell-laminin interactions promote cell adhesion and biosynthesis, which suggests a laminin-functionalized biomaterial may be useful for promoting or maintaining the NP cell phenotype. Here, a photocrosslinkable poly(ethylene glycol)-laminin 111 (PEG-LM111) hydrogel was developed. PEG-LM111 hydrogel mechanical properties could be tuned within the range of dynamic shear moduli values previously reported for human NP. When primary immature porcine NP cells were seeded onto PEG-LM111 hydrogels of varying stiffnesses, LM111 presenting hydrogels were found to promote cell clustering and increased levels of sGAG production as compared to stiffer LM111 presenting and PEG-only gels. When cells were encapsulated in 3D gels, hydrogel formulation was found to influence NP cell metabolism and expression of proposed NP phenotypic markers, with higher expression of N-cadherin and cytokeratin 8 observed for cells cultured in softer (<1 kPa) PEG-LM111 hydrogels. Overall, these findings suggest that soft, LM111 functionalized hydrogels may promote or maintain the expression of specific markers characteristic of an immature NP cell phenotype.
登录
查看更多内容
DOI：
10.1016/s0006-291x(02)00314-5
发表时间：
2002-05-10
影响因子：
3.1
作者：
Chen, J;Baer, AE;Setton, LA
通讯作者：
Setton, LA
影响因子：
2.5
作者：
Choi, Kyung-Suk;Cohn, Martin J.;Harfe, Brian D.
通讯作者：
Harfe, Brian D.
影响因子：
56.9
作者：
Cukierman, E;Pankov, R;Yamada, KM
通讯作者：
Yamada, KM
影响因子：
3
作者：
Bibby, SRS;Jones, DA;Urban, JPG
通讯作者：
Urban, JPG
影响因子：
7
作者：
Chou AI;Akintoye SO;Nicoll SB
通讯作者：
Nicoll SB