Improvement and optimization of standards for a preclinical animal test model of laser induced choroidal neovascularization.
Improvement and optimization of standards for a preclinical animal test model of laser induced choroidal neovascularization.
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激光脉络膜新生血管临床前动物试验模型标准的改进和优化
DOI：
10.1371/journal.pone.0094743
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Xie B
中科院分区：
文献类型：
--
作者：
Zhu Y;Lu Q;Shen J;Zhang L;Gao Y;Shen X;Xie B
由于激光诱导脉络膜新生血管（CNV）的小鼠模型正在成为全球研究 CNV 发病机制及其对治疗反应最成熟和最常用的模型，具体操作标准尚未明确。本研究的目的是比较不同年龄、性别、CNV过程持续时间和激光光斑治疗位置的小鼠的CNV病变大小，以提出可能改善和优化模型质量的建议。对不同年龄的C57/BL6小鼠每眼进行二极管激光光凝治疗，并在不同观察时间灌注含有荧光素标记葡聚糖的PBS。然后通过荧光显微镜检查脉络膜平片以测量 CNV 面积。通过实时 RT-PCR 检测分析 5-8 周和 16-20 周龄雄性和雌性 C57BL/6 小鼠眼杯中几种血管生成细胞因子的信使-RNA 表达水平。结果显示，与雄性小鼠相比，雌性小鼠眼中的 CNV 面积明显更大，并且多种血管生成细胞因子的表达水平升高。 16-20周龄雌性小鼠的CNV面积最大。光凝后第14天CNV平均面积显着增加。与远离视盘的 2PD 或 3PD 激光点相比，远离视盘的 1PD 激光点诱发了最大的 CNV 面积。在彼此距离小于 1PD 的激光光斑中观察到 NV 的相互作用。目前的结果表明，16-20 周龄的雌性 C57BL/6 小鼠出现了最明显的 CNV 病变大小，激光光斑从视盘传递 1PD。观察分析的最佳时间是光凝术后第14天。
As the murine model of laser-induced choroidal neovascularization (CNV) is becoming the most established and commonly utilized model worldwide for studying the pathogenesis of CNV and its response to treatment, specific operating standards are yet to be clarified. The purpose of this study is to compare the lesion size of CNV in mice with different ages, sex, durations of CNV process, and treated positions of laser spots, to make recommendations that may improve and optimize the quality of the model. C57/BL6 mice of different ages were treated with diode laser photocoagulation per eye and perfused with PBS containing fluorescein-labeled dextran at different time of observation. Choroid flat mounts, were then examined by fluorescence microscopy for the measurement of CNV area. Messenger-RNA expression levels of several angiogenic cytokines in eye cups of male and female C57BL/6 mice at 5–8 and 16–20 week-old were analyzed by real-time RT-PCR assay. The results showed significantly more CNV area in eyes of female mice compared to male mice with the expression level of several angiogenic cytokines elevated. 16–20-week-old female mice developed the biggest area of CNV. The mean area of CNV increased significantly at the 14th day after photocoagulation. Laser spots delivered 1PD away from the optic disc induced the biggest area of CNV compared to those 2PD or 3PD away. Interaction of NV was observed in laser spots delivered less than 1PD away from each other. The current results suggest that 16–20-week-old female C57BL/6 mice developed the most distinct CNV lesion size with laser spots delivered 1PD away from the optic disc. The best time to observe and analyze is the 14th day after photocoagulation.
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影响因子：
15.9
作者：
Ashcroft, GS;Mills, SJ
通讯作者：
Mills, SJ
影响因子：
4.2
作者：
FREUND, KB;YANNUZZI, LA;SORENSON, JA
通讯作者：
SORENSON, JA
影响因子：
--
作者：
Albert, Daniel M.;Neekhra, Aneesh;Wang, Shoujian;Darjatmoko, Soesiawati R.;Sorenson, Christine M.;Dubielzig, Richard R.;Sheibani, Nader
通讯作者：
Sheibani, Nader
影响因子：
--
作者：
Feng, Yuxi;Pfister, Frederick;Hammes, Hans-Peter
通讯作者：
Hammes, Hans-Peter
影响因子：
5.6
作者：
Ando, A;Yang, A;Campochiaro, PA
通讯作者：
Campochiaro, PA