Antimicrobial effects of nanofiber poly(caprolactone) tissue scaffolds releasing rifampicin.
Antimicrobial effects of nanofiber poly(caprolactone) tissue scaffolds releasing rifampicin.
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DOI：
10.1007/s10856-012-4609-3
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发表时间：
2012-06
影响因子：
3.7
通讯作者：
Popat, Ketul C.
中科院分区：
文献类型：
--
作者：
Ruckh, Timothy T.;Oldinski, Rachael A.;Carroll, Derek A.;Mikhova, Krasimira;Bryers, James D.;Popat, Ketul C.
本研究在体外静态条件下量化了利福平（rifampicin， RIF）对革兰氏阳性菌和革兰氏阴性菌的抗生素释放动力学和随后的杀菌效果。采用10%或20% (w/w) RIF的静电纺丝聚己内酯（PCL）制备载抗生素支架。表征了支架纤维直径和RIF载荷，并测量了RIF释放动力学。用铜绿假单胞菌和表皮葡萄球菌分别接种rif释放和不rif支架，荧光显微镜检测活菌和死菌的悬浮浓度。用扫描电镜检查附着菌和生物膜的形成。无RIF的平均纤维直径为557±399 nm， 10% RIF的平均纤维直径为402±225 nm， 20% RIF的平均纤维直径为665±402 nm。RIF释放动力学表现为在第一个小时内的短爆发释放，随后是7小时的零级释放，在此期间，两种RIF支架释放了约50%的初始RIF质量载荷。暴露于对照支架时，铜绿假单胞菌和表皮假单胞菌悬浮细胞群按照对数生长模型增殖；然而，两种含rif的支架完全抑制了悬浮液中的细菌生长，随后在前6小时内阻止了支架内生物膜的形成。
This study quantified the antibiotic release kinetics and subsequent bactericidal efficacy of rifampicin (RIF) against Gram-positive and Gram-negative bacteria under in vitro static conditions. Antibiotic-loaded scaffolds were fabricated by electrospinning poly(caprolactone) (PCL) with 10% or 20% (w/w) RIF. Scaffold fiber diameter and RIF loading were characterized, and RIF release kinetics were measured. RIF-releasing and RIF-free scaffolds were inoculated with Pseudomonas aeruginosa and Staphylococcus epidermidis, and the suspended concentration live and dead bacteria were determined by fluorescent microscopy. Adherent bacteria and biofilm formation were examined using scanning electron microscopy. Mean fiber diameters were 557 ± 399 nm for RIF-free, 402 ± 225 nm for 10% RIF, and 665 ± 402 nm for 20% RIF scaffolds. RIF release kinetics exhibited a short-burst release during the first hour, followed by a 7 h, zero-order release during which both RIF scaffolds released ~50% of their initial RIF mass loading. P. aeruginosa and S. epidermidis suspended cell populations proliferated in accordance with logarithmic growth models when exposed to control scaffolds; however both RIF-containing scaffolds completely inhibited bacterial growth in suspension and, subsequently, prevented biofilm formation within the scaffolds through the first 6 h.
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影响因子：
14
作者：
Bashur, Chris A.;Dahlgren, Linda A.;Goldstein, Aaron S.
通讯作者：
Goldstein, Aaron S.
影响因子：
4.1
作者：
Guarino, Vincenzo;Taddei, Paola;Ambrosio, Luigi
通讯作者：
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影响因子：
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作者：
Bjerketorp, J;Jacobsson, K;Frykberg, L
通讯作者：
Frykberg, L
DOI：
10.1016/j.mrfmmm.2009.10.015
发表时间：
2010-01-05
影响因子：
2.3
作者：
Jatsenko, Tatjana;Tover, Andres;Kivisaar, Maia
通讯作者：
Kivisaar, Maia
影响因子：
120.7
作者：
Klevens, R. Monina;Morrison, Melissa A.;Fridkin, Scott K.
通讯作者：
Fridkin, Scott K.