Circulating tumour markers can define patients with normal colons, benign polyps, and cancers.
Circulating tumour markers can define patients with normal colons, benign polyps, and cancers.
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DOI：
10.1038/bjc.2011.230
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发表时间：
2011-07-12
影响因子：
8.8
通讯作者：
Cree, I. A.
中科院分区：
文献类型：
--
作者：
Mead, R.;Duku, M.;Bhandari, P.;Cree, I. A.
早期诊断是治愈结直肠癌的最佳机会。目前的筛查方案使用粪便潜血检测进行筛查，其灵敏度有限，特异性差。在这项研究中，我们研究了一系列先前描述的利用循环游离DNA（cfDNA）的诊断标记物，其制备方法允许分离小DNA片段。从85例患者中提取循环游离DNA，其中包括35例无内镜异常的患者，26例良性结直肠腺瘤患者和24例结直肠癌患者。在每种情况下，对Line1 79 bp、Line1 300 bp、Alu 115 bp、Alu 247 bp和线粒体引物进行聚合酶链反应（PCR）。ELISA法检测癌胚抗原（CEA）。在正常、息肉和癌症人群之间分析每个标记物，并通过逻辑回归组合分析表现最好的标记物。最佳模型能够使用三种DNA标记物和CEA区分正常人群与腺瘤或癌，显示受试者操作特征（ROC）曲线下面积为0.855，息肉和癌症诊断的阳性预测值为81.1%。这些循环标志物与其他标志物的组合提供了一个简单的血液检查作为可能的二次筛查结直肠癌和息肉的患者粪便潜血试验阳性的前景。
Early diagnosis represents the best opportunity for cure of colorectal cancer. Current screening programmes use faecal occult blood testing for screening, which has limited sensitivity and poor specificity. In this study we looked at a series of previously described diagnostic markers utilising circulating free DNA (cfDNA), with a preparation method allowing small DNA fragments to be isolated. The Circulating free DNA was isolated from samples obtained from 85 patients, including 35 patients without endoscopic abnormality, a group of 26 patients with benign colorectal adenomas, and 24 patients with colorectal carcinomas. In each case, polymerase chain reaction (PCR) was performed for Line1 79 bp, Line1 300 bp, Alu 115 bp, Alu 247 bp, and mitochondrial primers. In addition, carcinoembryonic antigen (CEA) was measured by ELISA. Each marker was analysed between normal, polyp, and cancer populations, and the best performing analysed in combination by logistic regression. The best model was able to discriminate normal from populations with adenoma or carcinoma using three DNA markers and CEA, showing an area under the receiver operator characteristic (ROC) curve of 0.855 with a positive predictive value of 81.1% for polyps and cancer diagnosis. These circulating markers in combination with other markers offer the prospect of a simple blood test as a possible secondary screen for colorectal cancers and polyps in patients with positive faecal occult blood tests.
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影响因子：
4.7
作者：
Hosgood, H. Dean, III;Liu, Chin-San;Lan, Qing
通讯作者：
Lan, Qing
影响因子：
4.5
作者：
Ellinger, Joerg;Albers, Peter;Bastian, Patrick J.
通讯作者：
Bastian, Patrick J.
影响因子：
3.1
作者：
Catarino, Raquel;Ferreira, Maria M.;Medeiros, Rui
通讯作者：
Medeiros, Rui
DOI：
10.1196/annals.1448.035
发表时间：
2008-01-01
期刊：
CIRCULATING NUCLEIC ACIDS IN PLASMA AND SERUM V
影响因子：
--
作者：
Kirsch, Christoph;Weickmann, Sabine;Fleischhacker, Michael
通讯作者：
Fleischhacker, Michael
DOI：
10.1073/pnas.0507904102
发表时间：
2005-11-08
影响因子：
11.1
作者：
Diehl, F;Li, M;Vogelstein, B
通讯作者：
Vogelstein, B