No skin off your back: the sampling and extraction of sebum for metabolomics.
No skin off your back: the sampling and extraction of sebum for metabolomics.
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DOI：
10.1007/s11306-023-01982-3
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发表时间：
2023-03-24
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影响因子：
--
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中科院分区：
文献类型：
--
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基于皮脂的代谢组学（“皮脂学”的子集）是一个发展中的领域，其涉及在人类皮脂中发现的代谢物的采样、鉴定和定量。皮脂是由皮脂腺分泌到皮肤表面上的富含脂质的油性物质，用于皮肤稳态、润滑、温度调节和环境保护。在过去的十年中，由于其在一系列临床和环境应用的非侵入性采样后的快速分析潜力，对皮脂的兴趣不断增长。提供各种皮脂采样技术及其相关挑战的概述。评估皮脂在临床研究、药物监测和人体生物监测中的应用。对将来使用皮脂作为诊断生物流体的机会进行评论。于2023年1月12日使用Scopus搜索引擎对1960年至2022年期间有关皮肤表面分析的选定关键词进行了文献计量分析。已发表的文献根据工作在以下领域的贡献进行了划分：对皮脂的理解，其组成，使用的分析技术或使用皮脂的目的。本综述总结了研究结果。历史上，大约有15种采样方法用于皮脂收集。样品制备方法根据感兴趣的分析物而异，并进行了总结。皮脂的使用不仅限于皮肤疾病或药物监测，还可用于其他全身性疾病。大多数与皮脂相关的代谢物的非靶向分析工作是在最近二十年进行的。皮脂具有超越皮肤研究的巨大潜力，并且了解一个人的生理状态如何通过皮脂腺本身或通过与皮脂分泌的相互作用影响或反映在皮肤代谢组上，将为更简单的生物监测打开大门。皮脂作为环境代谢物的汇，具有有待探索的应用，例如生物安全，跨境迁移，局部暴露于有害物质和高通量人群筛查。随着挥发性顶空和脂质组学方法开发的快速进展以及代谢组学社区更好地注释未知物种的能力，这些应用将成为可能。皮肤表面分析的一个关键问题是在有意义的生物学解释之前确定皮肤表面上发现的代谢物的来源。
Sebum-based metabolomics (a subset of “sebomics”) is a developing field that involves the sampling, identification, and quantification of metabolites found in human sebum. Sebum is a lipid-rich oily substance secreted by the sebaceous glands onto the skin surface for skin homeostasis, lubrication, thermoregulation, and environmental protection. Interest in sebomics has grown over the last decade due to its potential for rapid analysis following non-invasive sampling for a range of clinical and environmental applications. To provide an overview of various sebum sampling techniques with their associated challenges. To evaluate applications of sebum for clinical research, drug monitoring, and human biomonitoring. To provide a commentary of the opportunities of using sebum as a diagnostic biofluid in the future. Bibliometric analyses of selected keywords regarding skin surface analysis using the Scopus search engine from 1960 to 2022 was performed on 12th January 2023. The published literature was compartmentalised based on what the work contributed to in the following areas: the understanding about sebum, its composition, the analytical technologies used, or the purpose of use of sebum. The findings were summarised in this review. Historically, about 15 methods of sampling have been used for sebum collection. The sample preparation approaches vary depending on the analytes of interest and are summarised. The use of sebum is not limited to just skin diseases or drug monitoring but also demonstrated for other systemic disease. Most of the work carried out for untargeted analysis of metabolites associated with sebum has been in the recent two decades. Sebum has a huge potential beyond skin research and understanding how one’s physiological state affects or reflects on the skin metabolome via the sebaceous glands itself or by interactions with sebaceous secretion, will open doors for simpler biomonitoring. Sebum acts as a sink to environmental metabolites and has applications awaiting to be explored, such as biosecurity, cross-border migration, localised exposure to harmful substances, and high-throughput population screening. These applications will be possible with rapid advances in volatile headspace and lipidomics method development as well as the ability of the metabolomics community to annotate unknown species better. A key issue with skin surface analysis that remains unsolved is attributing the source of the metabolites found on the skin surface before meaningful biological interpretation.
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影响因子：
3.6
作者：
Cho YT;Su H;Wu CY;Jeng J;Lee CW;Wu DC;Huang TL;Shiea J
通讯作者：
Shiea J
DOI：
10.1073/pnas.1424409112
发表时间：
2015-04-28
影响因子：
11.1
作者：
Bouslimani, Amina;Porto, Carla;Dorrestein, Pieter C.
通讯作者：
Dorrestein, Pieter C.
影响因子：
3.7
作者：
Humphreys, Devon P.;Gavin, Kathleen M.;Olds, Kaylan M.;Bonaca, Marc P.;Bauer, Timothy A.
通讯作者：
Bauer, Timothy A.
影响因子：
2.7
作者：
Clarys, P;Barel, A
通讯作者：
Barel, A
影响因子：
10.3
作者：
BURTON, JL;CUNLIFFE, WJ;SHUSTER, S
通讯作者：
SHUSTER, S