Characterization of bacterial microbiota compositions along the intestinal tract in pigs and their interactions and functions.
Characterization of bacterial microbiota compositions along the intestinal tract in pigs and their interactions and functions.
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DOI：
10.1038/s41598-018-30932-6
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发表时间：
2018-08-24
影响因子：
4.6
通讯作者：
Folch JM
中科院分区：
文献类型：
--
作者：
Crespo-Piazuelo D;Estellé J;Revilla M;Criado-Mesas L;Ramayo-Caldas Y;Óvilo C;Fernández AI;Ballester M;Folch JM
除了在肉类生产方面的价值外，由于其生理上的相似性，猪是人类消化道研究的有趣动物模型。本研究的目的是描述伊比利亚猪肠道微生物组的组成、分布和相互作用及其在全身能量稳态中的作用。从13头去势公猪的5个肠段（十二指肠、空肠、回肠、结肠近端和远端）的微生物组中扩增出16S rRNA基因V3-V4区并测序。在所有样本中共发现1669个操作分类单位，分布于179个属。在小肠中最丰富的两个属是乳酸杆菌和梭状芽孢杆菌，而在结肠中占优势的是普雷沃氏菌。猪的结肠样本比小肠样本更相似，种类也更丰富。在小肠中，宏基因组预测指出了微生物增殖和维持所需的可用简单碳水化合物的快速内化和转化。在结肠中，厌氧菌之间存在降解植物多糖产生短链脂肪酸的竞争。这项研究证实，肠道微生物组的能量途径在其部分不同，并提供了属之间相关性的描述。
In addition to its value in meat production, the pig is an interesting animal model for human digestive tract studies due to its physiological similarities. The aim of this study was to describe the microbiome composition, distribution and interaction along the Iberian pig intestinal tract and its role in whole-body energy homeostasis. The V3-V4 region of the 16S rRNA gene was amplified and sequenced from the microbiomes of five gut sections (duodenum, jejunum, ileum, and proximal and distal colon) in thirteen castrated male pigs. A total of 1,669 operational taxonomic units distributed in 179 genera were found among all samples. The two most abundant genera in the small intestine were Lactobacillus and Clostridium, while Prevotella was predominant in the colon. The colon samples were more similar among the pigs and richer in species than the small intestine samples were. In the small intestine, the metagenome prediction pointed to rapid internalization and conversion of the available simple carbohydrates for microbial proliferation and maintenance. In the colon, a competition among anaerobic bacteria for plant polysaccharide degradation to produce short chain fatty acids was found. This study confirms that the energy pathways of the gut microbiome differ along its sections and provides a description of the correlations between genera.
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DOI：
10.1038/nrmicro3552
发表时间：
2016-01
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Donaldson GP;Lee SM;Mazmanian SK
通讯作者：
Mazmanian SK
影响因子：
14.9
作者：
Klindworth A;Pruesse E;Schweer T;Peplies J;Quast C;Horn M;Glöckner FO
通讯作者：
Glöckner FO
影响因子：
3.3
作者：
Camarinha-Silva, Amelia;Maushammer, Maria;Bennewitz, Joern
通讯作者：
Bennewitz, Joern
影响因子：
3.6
作者：
Ivarsson, E.;Roos, S.;Lindberg, J. E.
通讯作者：
Lindberg, J. E.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y