Expression of netrin-1 receptors in retina of oxygen-induced retinopathy in mice.
Expression of netrin-1 receptors in retina of oxygen-induced retinopathy in mice.
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DOI：
10.1186/1471-2415-14-102
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发表时间：
2014-08-22
影响因子：
2
通讯作者：
Xia XB
中科院分区：
文献类型：
--
作者：
Liu D;Xiong SQ;Shang L;Tian XF;Yang J;Xia XB
据报道，Netrin-1可促进氧诱导性视网膜病变（OIR）中的视网膜新生血管形成。然而，netrin-1受体，这可能介导netrin-1的作用，在视网膜新生血管形成，还没有得到表征。在这项研究中，我们研究了netrin-1受体亚型的表达和相关的变化，在视网膜OIR小鼠。C57 BL/6 J小鼠暴露于75±2%的氧气5天，然后返回到正常的空气中，以诱导视网膜新生血管。采用逆转录聚合酶链反应（RT-PCR）和Western blot检测小鼠视网膜netrin-1受体亚型的表达。netrin-1受体亚型和isolectin B4的双重染色用于确定netrin-1受体亚型在视网膜中的位置。通过玻璃体内注射UNC 5 B shRNA质粒实现对视网膜新生血管的抑制。通过荧光素血管造影和视网膜切片中视网膜前新生血管核的定量来检查视网膜新生血管。RT-PCR结果显示，除UNC 5A外，netrin-1受体亚型UNC 5 B、UNC 5C、UNC 5D、DCC、neogenin和A2 b均在出生后17 d的OIR小鼠视网膜中表达。免疫印迹显示，只有UNC 5 B的表达显着增加的那一天，免疫荧光结果显示，只有UNC 5 B和再生蛋白表达在视网膜血管。用UNC 5 B shRNA质粒治疗OIR小鼠显著减少了新生血管簇和新生血管向内界膜的生长。UNC 5 B可能促进OIR小鼠的视网膜新生血管形成。
Netrin-1 has been reported to promote retinal neovascularization in oxygen-induced retinopathy (OIR). However, netrin-1 receptors, which may mediate netrin-1 action during retinal neovascularization, have not been characterized. In this study, we investigated netrin-1 receptor subtype expression and associated changes in the retinas of mice with OIR. C57BL/6J mice were exposed to 75±2% oxygen for 5 days and then returned to normal air to induce retinal neovascularization. Reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot were used to examine the expression of netrin-1 receptor subtypes in the mouse retinas. Double staining of netrin-1 receptor subtypes and isolectin B4 was used to determine the location of the netrin-1 receptor subtypes in the retinas. Inhibition of retinal neovascularization was achieved by UNC5B shRNA plasmid intravitreal injection. Retinal neovascularization was examined by fluorescein angiography and quantification of preretinal neovascular nuclei in retinal sections. RT-PCR results showed that, except for UNC5A, netrin-1 receptor subtypes UNC5B, UNC5C, UNC5D, DCC, neogenin, and A2b were all expressed in the retinas of OIR mice 17 days after birth. Western blots showed that only UNC5B expression was significantly increased on that day, and immunofluorescence results showed that only UNC5B and neogenin were expressed in retinal vessels. Treatment of OIR mice with the UNC5B shRNA plasmid dramatically reduced neovascular tufts and neovascular outgrowth into the inner limiting membrane. UNC5B may promote retinal neovascularization in OIR mice.
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DOI：
10.1007/978-0-387-76715-4_2
发表时间：
2007-01-01
期刊：
AXON GROWTH AND GUIDANCE
影响因子：
--
作者：
Moore, Simon W.;Tessier-Lavigne, Marc;Kennedy, Timothy E.
通讯作者：
Kennedy, Timothy E.
影响因子：
11.8
作者：
Castets, Marie;Coissieux, Marie-May;Mehlen, Patrick
通讯作者：
Mehlen, Patrick
影响因子：
16.2
作者：
Keleman, K;Dickson, BJ
通讯作者：
Dickson, BJ
影响因子：
4.6
作者：
Navankasattusas, Sutip;Whitehead, Kevin J.;Li, Dean Y.
通讯作者：
Li, Dean Y.
DOI：
10.1016/j.biocel.2006.11.009
发表时间：
2007-01-01
影响因子：
4
作者：
Cole, Stacey J.;Bradford, DanaKai;Cooper, Helen M.
通讯作者：
Cooper, Helen M.