Vaccination with live attenuated simian immunodeficiency virus causes dynamic changes in intestinal CD4+CCR5+ T cells.
Vaccination with live attenuated simian immunodeficiency virus causes dynamic changes in intestinal CD4+CCR5+ T cells.
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DOI：
10.1186/1742-4690-8-8
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发表时间：
2011-02-03
期刊：
影响因子：
3.3
通讯作者：
Stebbings R
中科院分区：
文献类型：
--
作者：
Li B;Berry N;Ham C;Ferguson D;Smith D;Hall J;Page M;Quartey-Papafio R;Elsley W;Robinson M;Almond N;Stebbings R
用减毒SIV活疫苗接种可以防止可检测到的野生型病毒感染。我们已经研究了靶细胞耗竭是否有助于观察到的保护。在用减毒活SIV接种后，在接种后第3、7、10、21和125天测定肠CD 4 + CCR 5 + T细胞（野生型SIV感染和破坏的早期靶标）的频率。在初始对照中，在LPL TTrM-1和IEL TTrM-2亚群中主要发现适度频率的肠CD 4 + CCR 5 + T细胞。在第3天，LPL和IEL CD 4 + CCR 5 + TEM细胞显著增加，而分化程度较低的亚群大大减少，与活化诱导的成熟一致。CCR 5表达在第7天仍然很高，尽管亚群平衡从CD 4 + CCR 5 + TEM转移到分化程度较低的TTrM-2细胞。肠道CD 4 + CCR 5 + T细胞的这种增加先于第10天测量的SIV RNA血浆负荷的峰值。在第10天，肠道CD 4 + CCR 5 + T细胞的消耗大于65.9%，但总体CD 4 + T细胞稳态由增加的CD 4 + CCR 5- T细胞维持。在第21天和第125天，检测到大量的肠道CD 4 + CCR 5-幼稚TN细胞，同时在第125天检测到大量增加的CD 4 + CCR 5 + LPL TTrM-2和IEL TEM细胞，但SIV RNA血浆载量仍然很低。减毒SIV活疫苗接种后，肠道CD 4 + CCR 5 + T细胞的增加与靶细胞耗竭（作为保护机制）无关。相反，肠道CD 4 + CCR 5 + T细胞的增加可能与减毒活SIV疫苗接种所提供的保护相关或起作用。
Vaccination with live attenuated SIV can protect against detectable infection with wild-type virus. We have investigated whether target cell depletion contributes to the protection observed. Following vaccination with live attenuated SIV the frequency of intestinal CD4+CCR5+ T cells, an early target of wild-type SIV infection and destruction, was determined at days 3, 7, 10, 21 and 125 post inoculation. In naive controls, modest frequencies of intestinal CD4+CCR5+ T cells were predominantly found within the LPL TTrM-1 and IEL TTrM-2 subsets. At day 3, LPL and IEL CD4+CCR5+ TEM cells were dramatically increased whilst less differentiated subsets were greatly reduced, consistent with activation-induced maturation. CCR5 expression remained high at day 7, although there was a shift in subset balance from CD4+CCR5+ TEM to less differentiated TTrM-2 cells. This increase in intestinal CD4+CCR5+ T cells preceded the peak of SIV RNA plasma loads measured at day 10. Greater than 65.9% depletion of intestinal CD4+CCR5+ T cells followed at day 10, but overall CD4+ T cell homeostasis was maintained by increased CD4+CCR5- T cells. At days 21 and 125, high numbers of intestinal CD4+CCR5- naive TN cells were detected concurrent with greatly increased CD4+CCR5+ LPL TTrM-2 and IEL TEM cells at day 125, yet SIV RNA plasma loads remained low. This increase in intestinal CD4+CCR5+ T cells, following vaccination with live attenuated SIV, does not correlate with target cell depletion as a mechanism of protection. Instead, increased intestinal CD4+CCR5+ T cells may correlate with or contribute to the protection conferred by vaccination with live attenuated SIV.
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影响因子：
4.4
作者：
Amyes, E;McMichael, AJ;Callan, MFC
通讯作者：
Callan, MFC
影响因子：
5.4
作者：
Barnett, Susan W.;Burke, Brian;Srivastava, Indresh K.
通讯作者：
Srivastava, Indresh K.
DOI：
10.1084/jem.20060134
发表时间：
2006-11-27
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Gauduin MC;Yu Y;Barabasz A;Carville A;Piatak M;Lifson JD;Desrosiers RC;Johnson RP
通讯作者：
Johnson RP
影响因子：
4.1
作者：
Friedrich, Thomas C.;Watkins, David I.
通讯作者：
Watkins, David I.
影响因子：
3.8
作者：
Anton, PA;Elliott, J;Giorgi, JV
通讯作者：
Giorgi, JV