Dynamics and Complexity of Dark Fermentation Microbial Communities Producing Hydrogen From Sugar Beet Molasses in Continuously Operating Packed Bed Reactors.
Dynamics and Complexity of Dark Fermentation Microbial Communities Producing Hydrogen From Sugar Beet Molasses in Continuously Operating Packed Bed Reactors.
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DOI：
10.3389/fmicb.2020.612344
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发表时间：
2020
影响因子：
5.2
通讯作者：
Sikora A
中科院分区：
文献类型：
--
作者：
Detman A;Laubitz D;Chojnacka A;Wiktorowska-Sowa E;Piotrowski J;Salamon A;Kaźmierczak W;Błaszczyk MK;Barberan A;Chen Y;Łupikasza E;Yang F;Sikora A
本研究描述了在五个填充床反应器（PBRs）中从甜菜糖蜜产生富氢发酵气体的微生物群落的动态和复杂性。这些生物反应器是从甜菜工业的副产品中生产氢气的系统的一部分，该系统一直在波兰的一家糖厂中连续运行。对不同工作容积、不同填料、不同结构、不同接种剂的PBS进行了试验。本研究的重点是分析（基于16 S rRNA分析和鸟枪宏基因组测序）的微生物群落选择的PBRs的条件下，高（>100 cm 3/g糖蜜COD）和低（<50 cm 3/g糖蜜COD）的产氢效率。产氢的稳定性和效率取决于暗发酵微生物群落的组成。测试样品之间最显著的差异是产氢菌与乳酸菌的比例。如分别通过16 S rRNA测序或鸟枪宏基因组测序所确定的，在HPB与LAB 12的比率为4：2.5或2.5：1时实现了最高的产氢效率（130-160 cm 3/g糖蜜COD）。最丰富的梭菌属是C. pasteurianum和C.酪丁酸菌LAB相对于HPB的多重优势（3：1-4：1）或梭菌相对于LAB的多重优势（5：1-60：1）导致产氢减少。通过短链脂肪酸和乙醇的过量产生来说明氢产生的抑制。此外，乙醇浓度可能是促进DF生物反应器中碳水化合物从产氢途径向乳酸发酵或产溶剂途径的代谢转变的相关标志物或因素。这项研究的新奇在于确定了产氢菌和乳酸菌之间的群落平衡，以实现稳定的产氢系统。这种平衡源于产氢微生物群落的长期选择、操作条件如生物反应器构建、填料、水力停留时间和底物浓度。通过对来自其他研究的暗发酵生物反应器中HPB和LAB之间的比例进行额外分析，证实了这一发现。这些结果有助于在关系和营养相互作用领域的知识进步，特别是在黑暗发酵微生物群落中细菌之间的乳酸盐交叉喂养。
This study describes the dynamics and complexity of microbial communities producing hydrogen-rich fermentation gas from sugar-beet molasses in five packed-bed reactors (PBRs). The bioreactors constitute a part of a system producing hydrogen from the by-products of the sugar-beet industry that has been operating continuously in one of the Polish sugar factories. PBRs with different working volumes, packing materials, construction and inocula were tested. This study focused on analysis (based on 16S rRNA profiling and shotgun metagenomics sequencing) of the microbial communities selected in the PBRs under the conditions of high (>100 cm3/g COD of molasses) and low (<50 cm3/g COD of molasses) efficiencies of hydrogen production. The stability and efficiency of the hydrogen production are determined by the composition of dark fermentation microbial communities. The most striking difference between the tested samples is the ratio of hydrogen producers to lactic acid bacteria. The highest efficiency of hydrogen production (130–160 cm3/g COD of molasses) was achieved at the ratios of HPB to LAB ≈ 4:2.5 or 2.5:1 as determined by 16S rRNA sequencing or shotgun metagenomics sequencing, respectively. The most abundant Clostridium species were C. pasteurianum and C. tyrobutyricum. A multiple predominance of LAB over HPB (3:1–4:1) or clostridia over LAB (5:1–60:1) results in decreased hydrogen production. Inhibition of hydrogen production was illustrated by overproduction of short chain fatty acids and ethanol. Furthermore, concentration of ethanol might be a relevant marker or factor promoting a metabolic shift in the DF bioreactors processing carbohydrates from hydrogen-yielding toward lactic acid fermentation or solventogenic pathways. The novelty of this study is identifying a community balance between hydrogen producers and lactic acid bacteria for stable hydrogen producing systems. The balance stems from long-term selection of hydrogen-producing microbial community, operating conditions such as bioreactor construction, packing material, hydraulic retention time and substrate concentration. This finding is confirmed by additional analysis of the proportions between HPB and LAB in dark fermentation bioreactors from other studies. The results contribute to the advance of knowledge in the area of relationships and nutritional interactions especially the cross-feeding of lactate between bacteria in dark fermentation microbial communities.
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DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
7.2
作者：
Detman, Anna;Chojnacka, Aleksandra;Sikora, Anna
通讯作者：
Sikora, Anna
影响因子：
1.8
作者：
Detman, Anna;Chojnacka, Aleksandra;Sikora, Anna
通讯作者：
Sikora, Anna
影响因子：
7.4
作者：
Garcia-Depraect, Octavio;Rene, Eldon R.;Leon-Becerril, Elizabeth
通讯作者：
Leon-Becerril, Elizabeth