Cyclic mechanical loading promotes bacterial penetration along composite restoration marginal gaps.
Cyclic mechanical loading promotes bacterial penetration along composite restoration marginal gaps.
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循环机械载荷促进沿复合恢复边缘间隙的细菌穿透。
DOI：
10.1016/j.dental.2015.03.011
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发表时间：
2015-06
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
继发龋是复合修复体置换最常见的原因，通常形成于牙本质与充填物之间。本研究的目的是利用一种新型的生物反应器系统和试样设计来研究循环加载和细菌暴露对牙本质和树脂复合修复材料界面间隙细菌渗透的综合影响。将人磨牙加工成3mm厚的盘状，并放置2mm深× 5mm直径的腔体，在其中放置复合修复体。沿着牙本质和修复体之间的一半界面引入一个~ 15-30微米（小）或~ 300微米宽（大）的牙本质修复间隙。变形链球菌UA 159生物膜在每个样品上生长，然后在生物反应器中进行循环加载和不循环加载的测试。两组样品均检测2周，检测后生物膜活力用活死法确认。将样品固定、安装并横切以显示间隙并观察细菌渗透的深度。结果表明，对于大间隙样品，细菌很容易渗透到间隙的全部深度，与加载或非加载条件无关。所有循环加载的小间隙样品的结果表明，细菌的渗透深度始终达到100%，而未加载的样品的平均渗透深度仅为67%，6个样品中只有2个达到100%。开发了一种新的生物反应器，可以将修复牙齿的循环机械载荷和细菌暴露相结合，用于细菌生物膜和脱矿研究。循环加载被证明有助于细菌渗透到狭窄的边缘间隙，最终可能促进继发性龋齿的形成。
Secondary caries is the most common reason for composite restoration replacement and usually forms between dentin and the filling. The objective of this study was to investigate the combined effect of cyclic loading and bacterial exposure on bacterial penetration into gaps at the interface between dentin and resin composite restorative material using a novel bioreactor system and test specimen design. Human molars were machined into 3 mm thick disks with 2 mm deep × 5 mm diameter cavity preparations into which composite restorations were placed. A ∼15-30 micrometer (small) or ∼300 micrometer wide (large) dentin-restoration gap was introduced along half of the interface between the dentin and restoration. Streptococcus mutans UA 159 biofilms were grown on each sample prior to testing in a bioreactor both with and without cyclic loading. Both groups of samples were tested for 2 weeks and post-test biofilm viability was confirmed with a live-dead assay. Samples were fixed, mounted and cross-sectioned to reveal the gaps and observe the depth of bacterial penetration. It was shown that for large gap samples the bacteria easily penetrated to the full depth of the gap independent of loading or non-loading conditions. The results for all cyclically loaded small gap samples show a consistently deep bacterial penetration down 100% of the gap while the average penetration depth was only 67% for the non-loaded samples with only two of six samples reaching 100%. A new bioreactor was developed that allows combining cyclic mechanical loading and bacterial exposure of restored teeth for bacterial biofilm and demineralization studies. Cyclic loading was shown to aid bacterial penetration into narrow marginal gaps, which could ultimately promote secondary caries formation.
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DOI：
10.2174/1874210601004010223
发表时间：
2010-12-15
期刊：
The open dentistry journal
影响因子：
--
作者：
Cho A;Suzuki S;Hatakeyama J;Haruyama N;Kulkarni AB
通讯作者：
Kulkarni AB
DOI：
10.1002/jbm.b.30338
发表时间：
2006-01-01
影响因子：
3.4
作者：
Lohbauer, U;Frankenberger, R;Petschelt, A
通讯作者：
Petschelt, A
影响因子：
3
作者：
Al-Ahmad, Ali;Wiedmann-Al-Ahmad, Margit;Arweiler, Nicole Birgit
通讯作者：
Arweiler, Nicole Birgit
影响因子：
2.4
作者：
Chen R;Rudney J;Aparicio C;Fok A;Jones RS
通讯作者：
Jones RS
影响因子：
4.2
作者：
Cenci, M. S.;Pereira-Cenci, T.;ten Cate, J. M.
通讯作者：
ten Cate, J. M.