Development and Characterization of a Novel Wheat-Tetraploid Thinopyrum elongatum 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage.
Development and Characterization of a Novel Wheat-Tetraploid Thinopyrum elongatum 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage.
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DOI：
10.3390/plants12122311
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发表时间：
2023-06-14
期刊：
影响因子：
--
通讯作者：
Kang H
中科院分区：
文献类型：
--
作者：
Gong B;Zhao L;Zeng C;Zhu W;Xu L;Wu D;Cheng Y;Wang Y;Zeng J;Fan X;Sha L;Zhang H;Chen G;Zhou Y;Kang H
条锈病是由小麦条锈菌（Pucciniastriiformis f.）小麦叶部病害是世界范围内危害小麦最严重的病害之一。培育持久抗病的小麦新品种是防治该病最有效的途径。四倍体长穗偃麦草（2n = 4x = 28，EEEE）具有抗条锈病、赤霉病和白粉病等多种病害的基因，是小麦品种改良的重要第三代遗传资源。在这里，一个新的小麦四倍体Th。利用基因组原位杂交和荧光原位杂交技术对长穗偃麦草6 E（6D）二体代换系（K17-1065-4）进行了染色体涂染分析。病害反应评价表明，K17-1065-4在成株期对条锈病具有高抗性。通过对二倍体Th.在6 E染色体上检测到3382个特异SSR序列。共开发了60个SSR标记，其中33个标记能准确追踪四倍体Th的6 E染色体。elongatum中的抗病基因，这些抗病基因与小麦遗传背景中的抗病基因连锁。分子标记分析表明，有10个标记可用于区分Th。与其他小麦相关的物种。因此，K17-1065-4是一个可用于抗病小麦品种选育的新种质。本研究所开发的分子标记可为四倍体小麦抗条锈病基因定位提供参考。长形的
Stripe rust, which is caused by Puccinia striiformis f. sp. tritici, is one of the most devastating foliar diseases of common wheat worldwide. Breeding new wheat varieties with durable resistance is the most effective way of controlling the disease. Tetraploid Thinopyrum elongatum (2n = 4x = 28, EEEE) carries a variety of genes conferring resistance to multiple diseases, including stripe rust, Fusarium head blight, and powdery mildew, which makes it a valuable tertiary genetic resource for enhancing wheat cultivar improvement. Here, a novel wheat–tetraploid Th. elongatum 6E (6D) disomic substitution line (K17-1065-4) was characterized using genomic in situ hybridization and fluorescence in situ hybridization chromosome painting analyses. The evaluation of disease responses revealed that K17-1065-4 is highly resistant to stripe rust at the adult stage. By analyzing the whole-genome sequence of diploid Th. elongatum, we detected 3382 specific SSR sequences on chromosome 6E. Sixty SSR markers were developed, and thirty-three of them can accurately trace chromosome 6E of tetraploid Th. elongatum, which were linked to the disease resistance gene(s) in the wheat genetic background. The molecular marker analysis indicated that 10 markers may be used to distinguish Th. elongatum from other wheat-related species. Thus, K17-1065-4 carrying the stripe rust resistance gene(s) is a novel germplasm useful for breeding disease-resistant wheat cultivars. The molecular markers developed in this study may facilitate the mapping of the stripe rust resistance gene on chromosome 6E of tetraploid Th. elongatum.
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影响因子：
3.1
作者：
Li, HJ;Chen, O;Chu, CC
通讯作者：
Chu, CC
影响因子：
5.4
作者：
Carter, Arron Hyrum;Chen, X. M.;Kidwell, K. K.
通讯作者：
Kidwell, K. K.
DOI：
10.3390/ma11071231
发表时间：
2018-07-18
期刊：
Materials (Basel, Switzerland)
影响因子：
--
作者：
Li D;Zheng ZL;Yang R;Zhang P
通讯作者：
Zhang P
影响因子：
56.9
作者：
Fu, Daolin;Uauy, Cristobal;Dubcovsky, Jorge
通讯作者：
Dubcovsky, Jorge
影响因子：
3.3
作者：
Han, Yonghua;Zhang, Tao;Jiang, Jiming
通讯作者：
Jiang, Jiming