Assessment of aflatoxin exposure, growth faltering and the gut microbiome among children in rural Guatemala: protocol for an observational prospective cohort and bioreactor simulations.
Assessment of aflatoxin exposure, growth faltering and the gut microbiome among children in rural Guatemala: protocol for an observational prospective cohort and bioreactor simulations.
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DOI：
10.1136/bmjpo-2023-001960
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发表时间：
2023-04
影响因子：
2.6
通讯作者：
中科院分区：
文献类型：
--
作者：
黄曲霉毒素B1（AFB 1）是由生长在玉米上的黄曲霉和寄生曲霉产生的一种致癌物质。鉴于低收入和中等收入国家儿童发育迟缓（即生长受损）和其他营养失调的高发病率，在这些国家，玉米消费，黄曲霉毒素暴露的作用可能是显着的。观察报告已经证明了黄曲霉毒素暴露与儿童生长发育受损之间的关联;然而，大多数报告都是横截面的，没有评估黄曲霉毒素的季节性变化、食物制备和生长发育的动态变化。缺乏关于黄曲霉毒素如何影响儿童生长的生物学机制数据。这项研究纳入了来自危地马拉农村的儿童的前瞻性队列，以评估（1）黄曲霉毒素暴露与儿童生长之间的时间关联，以及（2）黄曲霉毒素暴露，炎症和儿童生长之间肠道微生物组的可能介导。我们将前瞻性地评估185名6-9个月大的儿童在18个月内的黄曲霉毒素暴露和身高年龄差异轨迹。我们将评估黄曲霉毒素暴露水平和肠道和全身炎症的生物标志物。我们将检查每个儿童的粪便微生物组，并确定不同AFB 1暴露水平和儿童生长轨迹的关键物种和代谢途径。同时，我们将使用生物反应器，接种粪便，以研究肠道微生物组对不同水平的黄曲霉毒素B1暴露的反应。我们将监测关键微生物代谢产物和黄曲霉毒素B1生物转化产物，以研究营养代谢和肠道微生物组对黄曲霉毒素解毒/代谢的影响。最后，我们将使用通径分析来总结黄曲霉毒素暴露和肠道微生物组对儿童生长的影响。获得了亚利桑那州立大学机构审查委员会（IRB; IRB Y 00016799）和Wuqu' Kawoq/Maya Health Alliance IRB（WK-2022-003）的伦理批准。研究结果将在科学报告和同行评审出版物中传播。
Aflatoxin B1 (AFB1) is a carcinogen produced by Aspergillus flavus and Aspergillus parasiticus which grow on maize. Given the high prevalence of child stunting (ie, impaired growth) and other nutritional disorders in low-income and middle-income countries, where maize is consumed, the role of aflatoxin exposure may be significant. Observational reports have demonstrated associations between aflatoxin exposure and impaired child growth; however, most have been cross-sectional and have not assessed seasonal variations in aflatoxin, food preparation and dynamic changes in growth. Biological mechanistic data on how aflatoxin may exert an impact on child growth is missing. This study incorporates a prospective cohort of children from rural Guatemala to assess (1) temporal associations between aflatoxin exposure and child growth and (2) possible mediation of the gut microbiome among aflatoxin exposure, inflammation and child growth. We will prospectively evaluate aflatoxin exposure and height-for-age difference trajectories for 18 months in a cohort of 185 children aged 6–9 months at enrolment. We will assess aflatoxin exposure levels and biomarkers of gut and systemic inflammation. We will examine the faecal microbiome of each child and identify key species and metabolic pathways for differing AFB1 exposure levels and child growth trajectories. In parallel, we will use bioreactors, inoculated with faeces, to investigate the response of the gut microbiome to varying levels of AFB1 exposure. We will monitor key microbial metabolites and AFB1 biotransformation products to study nutrient metabolism and the impact of the gut microbiome on aflatoxin detoxification/metabolism. Finally, we will use path analysis to summarise the effect of aflatoxin exposure and the gut microbiome on child growth. Ethics approval was obtained from Arizona State University Institutional Review Board (IRB; STUDY00016799) and Wuqu’ Kawoq/Maya Health Alliance IRB (WK-2022-003). Findings will be disseminated in scientific presentations and peer-reviewed publications.
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影响因子：
2.3
作者：
Jolly PE;Mazariegos M;Contreras H;Balas N;Junkins A;Aina IO;Minott S;Wang M;Phillips TD
通讯作者：
Phillips TD
影响因子：
4.9
作者：
Miller AC;Rohloff P;Blake A;Dhaenens E;Shaw L;Tuiz E;Grandesso F;Mendoza Montano C;Thomson DR
通讯作者：
Thomson DR
影响因子：
3.7
作者：
Smith JW;Kroker-Lobos MF;Lazo M;Rivera-Andrade A;Egner PA;Wedemeyer H;Torres O;Freedman ND;McGlynn KA;Guallar E;Groopman JD;Ramirez-Zea M
通讯作者：
Ramirez-Zea M
影响因子：
--
作者：
Kroker-Lobos, Maria F.;Alvarez, Christian S.;Groopman, John D.
通讯作者：
Groopman, John D.
影响因子：
8.1
作者：
Hoffmann, Vivian;Jones, Kelly;Leroy, Jef L.
通讯作者：
Leroy, Jef L.