Two-dimensional and three-dimensional viability measurements of adult stem cells with optical coherence phase microscopy.
Two-dimensional and three-dimensional viability measurements of adult stem cells with optical coherence phase microscopy.
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使用光学相干相位显微镜对成体干细胞进行二维和三维活力测量。
DOI：
10.1117/1.3606561
复制
发表时间：
2011
影响因子：
3.5
通讯作者：
Bagnaninchi PO
中科院分区：
文献类型：
--
作者：
Bagnaninchi PO
细胞活力测定是细胞生物学的基本工具。它们评估样品中的健康细胞，并能够定量细胞对感兴趣试剂的反应。二维（2D）和三维（3D）细胞培养中需要非侵入性和无标记测定，以促进时程活力研究。细胞与基质距离变化引起的细胞微动已被证明是细胞活力的标志，具有电细胞-基质阻抗传感（ECIS）。在这项研究中，我们研究了光学相干相位显微镜（OCPM）是否能够报告2D和3D中成体干细胞的相位波动，这可能与细胞微动有关。OCPM是围绕Thorlabs引擎（λo= 930 nm）开发的，并集成在带有定制扫描头的倒置显微镜中。在Mesenpro RS培养基中培养人脂肪来源的干细胞（ADSC，Invitrogen），并接种在ECIS阵列、2D细胞培养皿或3D高度多孔微标聚合物支架中。ADSC微动通过ECIS分析证实。然后用OCPM在2D和3D中研究活的和固定的ADSC。不同条件下的相位波动存在显著差异。这项研究表明，OCPM可以在2D和3D微观结构中评估细胞活力。
Cell viability assays are essential tools for cell biology. They assess healthy cells in a sample and enable the quantification of cellular responses to reagents of interest. Noninvasive and label-free assays are desirable in two-dimensional (2D) and three-dimensional (3D) cell culture to facilitate time-course viability studies. Cellular micromotion, emanating from cell to substrate distance variations, has been demonstrated as a marker of cell viability with electric cell-substrate impedance sensing (ECIS). In this study we investigated if optical coherence phase microscopy (OCPM) was able to report phase fluctuations of adult stem cells in 2D and 3D that could be associated with cellular micromotion. An OCPM has been developed around a Thorlabs engine (λo= 930 nm) and integrated in an inverted microscope with a custom scanning head. Human adipose derived stem cells (ADSCs, Invitrogen) were cultured in Mesenpro RS medium and seeded either on ECIS arrays, 2D cell culture dishes, or in 3D highly porous microplotted polymeric scaffolds. ADSC micromotion was confirmed by ECIS analysis. Live and fixed ADSCs were then investigated in 2D and 3D with OCPM. Significant differences were found in phase fluctuations between the different conditions. This study indicated that OCPM could potentially assess cell vitality in 2D and in 3D microstructures.
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分化脂肪干细胞的光学相干相位显微镜和阻抗传感测量相结合
DOI：
--
发表时间：
2010
期刊：
BiOS
影响因子：
--
作者：
P. Bagnaninchi
通讯作者：
P. Bagnaninchi
影响因子：
1.9
作者：
Lue, Niyom;Choi, Wonshik;Feld, Michael S.
通讯作者：
Feld, Michael S.
影响因子：
3.6
作者：
Popescu, G;Deflores, LP;Feld, MS
通讯作者：
Feld, MS
影响因子：
1.7
作者：
Ripandelli, G;Coppe, A M;Stirpe, M
通讯作者：
Stirpe, M
影响因子：
3.6
作者：
Zhang J;Rao B;Yu L;Chen Z
通讯作者：
Chen Z