Metagenomic Analysis Reveals New Microbiota Related to Fiber Digestion in Pigs.
Metagenomic Analysis Reveals New Microbiota Related to Fiber Digestion in Pigs.
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宏基因组分析揭示了与猪纤维消化相关的新微生物群
DOI：
10.3389/fmicb.2021.746717
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发表时间：
2021
影响因子：
5.2
通讯作者：
Huang R
中科院分区：
文献类型：
--
作者：
Liu G;Li P;Hou L;Niu Q;Pu G;Wang B;Du T;Kim SW;Niu P;Li Q;Huang R
充分利用高纤维、低成本的农作物副产品，有助于缓解人畜争粮的局面。猪对膳食纤维的消化主要是通过大肠中的微生物发酵。为了揭示猪纤维消化相关的微生物区系，在相同的饲养条件下，采集了274头160日龄健康苏淮母猪的粪便样品，测定了中性洗涤纤维（NDF）和酸性洗涤纤维（ADF）的表观消化率。对苏淮猪NDF表观消化率极高和极低以及ADF表观消化率极高和极低的样品进行鸟枪法宏基因组测序。在菌种水平上，H_NDF组有62种微生物与高纤维消化率相关，H_ADF组有54种微生物与高纤维消化率相关。其中，毛螺菌科细菌3-1和Alistipes sp. CAG：514可能是与纤维消化相关的新型微生物。此外，我们还发现微生物中更丰富的GH5和GH48家族（纤维素降解）基因、GH 39和GH53家族（半纤维素降解）基因可能有助于提高猪的NDF表观消化率，而微生物中更丰富的GH 3和GH 9家族（纤维素降解）基因可能有助于提高猪的ADF表观消化率。AA 4家族基因的丰度在H_NDF和H_ADF组显著高于L_NDF和L_ADF组（P < 0.05）。H_NDF组中的3条途径和H_ADF组中的4条途径是降解非淀粉多糖的重要途径，其相对丰度分别显著高于L_NDF和L_ADF组。与低表观消化率组相比，高表观消化率组苏淮猪肠道菌群中与纤维消化相关的基因和菌群丰度较高，可能具有更强的纤维消化潜力。本研究揭示了高纤维表观消化率猪的肠道菌群特征和微生物基因功能，为进一步了解肠道菌群对纤维消化率的影响提供了理论依据和参考。
Making full use of high fiber and low-cost crop coproducts is helpful to alleviate the situation of people and livestock competing for crops. Digestion of dietary fibers in pigs is mainly through microbial fermentation in the large intestine. To reveal microbiota related to fiber digestion in pigs, fecal samples have been collected from 274 healthy female Suhuai pigs at 160 days of age under the same feeding conditions and have measured apparent neutral detergent fiber (NDF) and acid detergent fiber (ADF) digestibility. Samples from Suhuai pigs with extreme high and low apparent NDF digestibility and extreme high and low apparent ADF digestibility were subjected to shotgun metagenomic sequencing. At the species level, 62 microbial species in H_NDF group and 54 microbial species in H_ADF group were related to high fiber digestibility. Among them, Lachnospiraceae bacterium 3-1 and Alistipes sp. CAG:514 may be new types of microorganisms associated with fiber digestion. In addition, we found that more abundant GH5 and GH48 family (contribute to cellulose degradation) genes, GH39 and GH53 family (contribute to hemicellulose degradation) genes in microorganisms may contribute to the higher apparent NDF digestibility of pigs, and more abundant GH3 and GH9 family (contribute to cellulose degradation) genes in microorganisms may contribute to the higher apparent ADF digestibility of pigs. The abundance of AA4 family (helps in lignin degradation) genes in H_NDF and H_ADF groups was significantly higher than that in L_NDF and L_ADF groups, respectively (P < 0.05). Three pathways in H_NDF group and four pathways in H_ADF group are important pathways associated with degradation of non-starch polysaccharides, and their relative abundance is significantly higher than that in L_NDF and L_ADF groups, respectively. Gut microbiota of Suhuai pigs with high apparent fiber digestibility had higher abundance of genes and microbiota related to fiber digestion and may have stronger fiber digestion potential compared with low apparent fiber digestibility group. This study revealed that the characteristics of gut microbiota and microbial gene functions of pigs with high fiber apparent digestibility, which provided a theoretical basis and reference for further understanding the impact of gut microbiota on fiber digestibility of pigs.
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DOI：
10.1093/bioinformatics/bts565
发表时间：
2012-12-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Fu L;Niu B;Zhu Z;Wu S;Li W
通讯作者：
Li W
影响因子：
11
作者：
Gharechahi, Javad;Vahidi, Mohammad Farhad;Salekdeh, Ghasem Hosseini
通讯作者：
Salekdeh, Ghasem Hosseini
影响因子：
4.4
作者：
Jia, W.;Slominski, B. A.;Jones, O.
通讯作者：
Jones, O.
影响因子：
2.1
作者：
Grinberg, Inna Rozman;Yin, Guohua;Lamed, Raphael
通讯作者：
Lamed, Raphael
影响因子：
2
作者：
Hübener, K;Vahjen, W;Simon, O
通讯作者：
Simon, O