Auditory cortical areas activated by slow frequency-modulated sounds in mice.
Auditory cortical areas activated by slow frequency-modulated sounds in mice.
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DOI：
10.1371/journal.pone.0068113
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Shibuki K
中科院分区：
文献类型：
--
作者：
Honma Y;Tsukano H;Horie M;Ohshima S;Tohmi M;Kubota Y;Takahashi K;Hishida R;Takahashi S;Shibuki K
在小鼠听觉皮层的核心区域，物种特异性发声的调频成分比调频方向选择性的下限慢。为了识别对慢频调制有选择性的皮层区域，我们使用经颅黄蛋白荧光成像技术研究了小鼠听觉皮层的音调反应。为了区分对频率调制的响应与对恒定频率刺激的响应，我们重点研究了暂时重复和叠加FM扫描方向反转后的瞬态荧光变化。研究发现，脑带皮质区的超声场对方向反转有选择性响应。背后磁场（DP）也对逆转反应较弱。对于UF的反应，没有发现明显的张力映射，并且右侧UF的反应幅度明显大于左侧UF的反应幅度。FM扫描反应的半最大潜伏期在UF区比在初级听觉皮层（A1）或前听觉区（AAF）短。功能鉴定的UF和DP的示踪剂注射实验证实，这两个区域接受内侧膝状核（MG）背侧的传入输入。利用双光子显微镜对fura-2染色的UF神经元进行钙成像，证实了UF神经元对慢频调制的方向和方向反转都有选择性。这些结果有力地表明UF，可能还有DP，在小鼠中作为专门处理慢频率调制的皮质区域发挥作用。
Species-specific vocalizations in mice have frequency-modulated (FM) components slower than the lower limit of FM direction selectivity in the core region of the mouse auditory cortex. To identify cortical areas selective to slow frequency modulation, we investigated tonal responses in the mouse auditory cortex using transcranial flavoprotein fluorescence imaging. For differentiating responses to frequency modulation from those to stimuli at constant frequencies, we focused on transient fluorescence changes after direction reversal of temporally repeated and superimposed FM sweeps. We found that the ultrasonic field (UF) in the belt cortical region selectively responded to the direction reversal. The dorsoposterior field (DP) also responded weakly to the reversal. Regarding the responses in UF, no apparent tonotopic map was found, and the right UF responses were significantly larger in amplitude than the left UF responses. The half-max latency in responses to FM sweeps was shorter in UF compared with that in the primary auditory cortex (A1) or anterior auditory field (AAF). Tracer injection experiments in the functionally identified UF and DP confirmed that these two areas receive afferent inputs from the dorsal part of the medial geniculate nucleus (MG). Calcium imaging of UF neurons stained with fura-2 were performed using a two-photon microscope, and the presence of UF neurons that were selective to both direction and direction reversal of slow frequency modulation was demonstrated. These results strongly suggest a role for UF, and possibly DP, as cortical areas specialized for processing slow frequency modulation in mice.
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影响因子：
25
作者：
Bandyopadhyay, Sharba;Shamma, Shihab A.;Kanold, Patrick O.
通讯作者：
Kanold, Patrick O.
DOI：
10.1111/j.1601-183x.2010.00570.x
发表时间：
2010-06-01
期刊：
Genes, brain, and behavior
影响因子：
--
作者：
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通讯作者：
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影响因子：
2.5
作者：
Llano, D. A.;Theyel, B. B.;Issa, N. P.
通讯作者：
Issa, N. P.
影响因子：
1.4
作者：
MIKAELIAN, DO;WARFIELD, D;NORRIS, O
通讯作者：
NORRIS, O
DOI：
10.1073/pnas.0907873107
发表时间：
2010-01-05
影响因子：
11.1
作者：
Lee, Charles C.;Sherman, S. Murray
通讯作者：
Sherman, S. Murray