Surfce Functionalized via AdLAMA3 Multilayer Coating for Re-epithelization Around Titanium Implants
Surfce Functionalized via AdLAMA3 Multilayer Coating for Re-epithelization Around Titanium Implants
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通过 AdLAMA3 多层涂层进行表面功能化，用于钛植入物周围的上皮再生
DOI：
10.3389/fbioe.2020.00624
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发表时间：
2020-06
影响因子：
5.7
通讯作者：
Yang Guoli
中科院分区：
文献类型：
--
作者：
Zhang Jing;Li Yongzheng;Li Jialu;Shi Yuan;Hu Jinxing;Yang Guoli
种植体周围上皮（PIE）在口腔环境和种植体表面之间形成关键的密封。与交界上皮（JE）相比，PIE的生物学封闭性较弱，界面上部缺少半桥粒（HDs）和内部基底层（含层粘连蛋白332的细胞外基质，IBL）。本研究旨在制备一种生物相容性好、能负载LAMA 3重组腺病毒载体（AdLAMA 3）的涂层，以促进PIE的再上皮化。通过层层组装技术和抗体-抗原特异性结合建立了AdLAMA 3功能化的钛表面。对PIE的细胞粘附和再上皮化等生物学特性进行了研究。体外实验结果表明，AdLAMA 3涂层可促进上皮细胞的早期粘附和细胞铺展。体内实验表明，AdLAMA 3涂层具有促进PIE愈合的作用，并能促进层粘连蛋白α3的表达和半桥粒的形成。这项研究可能为LAMA 3与钛表面的精确附着提供了一种新的方法和实验证据。该工艺可促进PIE的再上皮化。
The peri-implant epithelium (PIE) forms a crucial seal between the oral environment and the implant surface. Compared with the junctional epithelium (JE), the biological sealing of PIE is fragile, which lacks hemidesmosomes (HDs) and internal basal lamina (extracellular matrix containing laminin332, IBL) on the upper part of the interface. In the study, we aim to prepare a coating with good biocompatibility and ability to immobilize the recombinant adenovirus vector of LAMA3 (AdLAMA3) for promoting the re-epithelization of PIE. The titanium surface functionalized with AdLAMA3 was established via layer-by-layer assembly technique and antibody-antigen specific binding. The biological evaluations including cell adhesion and the re-epithelization of PIE were investigated. The results in vitro demonstrated that the AdLAMA3 coating could improve epithelial cell attachment and cell spreading in the early stage. In vivo experiments indicated that the AdLAMA3 coating on the implant surface has the potential to accelerate the healing of the PIE, and could promote the expression of laminin α3 and the formation of hemidesmosomes. This study might provide a novel approach and experimental evidence for the precise attachment of LAMA3 to titanium surfaces. The process could improve the re-epithelization of PIE.
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DOI：
--
发表时间：
1997-08
期刊：
The Bulletin of Tokyo Dental College
影响因子：
--
作者：
Takashi Inoue;T. Takeda;Lee Cy;Y. Abiko;Y. Ayukawa;T. Tanaka;Masao Yoshinari;Masaki Shimono
通讯作者：
Takashi Inoue;T. Takeda;Lee Cy;Y. Abiko;Y. Ayukawa;T. Tanaka;Masao Yoshinari;Masaki Shimono
影响因子：
3.5
作者：
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通讯作者：
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DOI：
10.5037/jomr.2016.7308
发表时间：
2016-07
期刊：
Journal of oral & maxillofacial research
影响因子：
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作者：
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通讯作者：
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DOI：
10.1016/j.jconrel.2016.05.031
发表时间：
2016-10-28
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
--
作者：
Kim N;Duncan GA;Hanes J;Suk JS
通讯作者：
Suk JS
影响因子：
6.9
作者：
Rousselle, Patricia;Montmasson, Marine;Garnier, Cecile
通讯作者：
Garnier, Cecile