A flow cytometry-based assay to determine the phagocytic activity of both clinical and nonclinical antibody samples against Chlamydia trachomatis.
A flow cytometry-based assay to determine the phagocytic activity of both clinical and nonclinical antibody samples against Chlamydia trachomatis.
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DOI：
10.1002/cyto.a.23353
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发表时间：
2018-05
期刊：
影响因子：
--
通讯作者：
Dietrich J
中科院分区：
文献类型：
--
作者：
Grasse M;Rosenkrands I;Olsen A;Follmann F;Dietrich J
在全球范围内，估计每年有1.31亿例新的衣原体感染病例。沙眼衣原体感染可对女性输卵管造成永久性损伤，导致不孕和异位妊娠的风险。非常需要针对沙眼衣原体的疫苗，因此需要用于评价功能性免疫应答的测定以用于未来的临床试验和流行病学研究。抗体在抵抗感染中起着至关重要的作用，并且可以通过几种功能（包括吞噬作用和中和作用）来保护。疫苗开发可以大大受益于测量功能性C的方法。沙眼特异性抗体在大量的样本。在目前的体外抗体保护测定中，其测量抗体促进C.对于沙眼衣原体，吞噬的细菌必须手动计数。这既需要劳动力，又耗时，并且它阻止了这种方法的高通量使用。因此，在本研究中，我们开发了一种简单快速的基于流式细胞术的测定方法，以测量抗体介导Fc受体依赖性吞噬作用的能力。该方法重现性高，适用于分析大量临床和非临床样品。版权所有© 2018作者.细胞计数A部分由Wiley Periodicals，Inc.出版。关于ISAC
Globally, an estimated 131 million new cases of chlamydial infection occur annually. Chlamydia trachomatis infection can cause permanent damage to the fallopian tubes in woman, resulting in infertility and a risk of ectopic pregnancy. There is a great need for a vaccine against Chlamydia trachomatis and as a result there is a need for assays to evaluate functional immune responses for use in future clinical trials and epidemiological studies. Antibodies play a crucial role in the defense against infection and can be protective by several functions, including phagocytosis and neutralization. Vaccine development could greatly benefit from a method to measure functional C. trachomatis‐specific antibodies in a large number of samples. In the current in vitro antibody protection assays, which measure the capacity of antibodies to facilitate phagocytic uptake of C. trachomatis, the phagocytosed bacteria have to be counted manually. This is both labor demanding, time consuming, and it prevents high‐throughput usage of this method. In this study, we, therefore, developed a simple and rapid flow cytometry based assay to measure the capacity of antibodies to mediate Fc‐receptor dependent phagocytosis. This method is highly reproducible and suitable to analyze large numbers of clinical and nonclinical samples. © 2018 The Authors. Cytometry Part A Published by Wiley Periodicals, Inc. on behalf of ISAC.
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DOI：
10.1152/ajplung.00440.2003
发表时间：
2004-08-01
影响因子：
4.9
作者：
Oberley, RE;Ault, KA;Snyder, JM
通讯作者：
Snyder, JM
影响因子：
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作者：
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影响因子：
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作者：
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影响因子：
3.7
作者：
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通讯作者：
Meurens, F.
影响因子：
6.4
作者：
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