A Cre knockin mouse reveals specific expression of Agouti gene in mesenchymal lineage cells in multiple organs and provides a unique tool for conditional gene targeting
A Cre knockin mouse reveals specific expression of Agouti gene in mesenchymal lineage cells in multiple organs and provides a unique tool for conditional gene targeting
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Cre敲入小鼠揭示了Agouti基因在多个器官的间充质谱系细胞中的特异性表达，并为条件基因靶向提供了独特的工具
DOI：
10.1007/s11248-023-00334-0
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发表时间：
2023-01
影响因子：
3
通讯作者：
Xin-Hua Liao
中科院分区：
文献类型：
--
作者：
Xing-Ru Shen;He-Li Zhang;Xu-Bo Zhao;Yang-Ge Wang;Xiao-Yang Tan;Lipeng Gao;Ruilin Sun;Xin-Hua Liao
老鼠的Agglutinase基因编码一种旁分泌信号因子，促进黑素细胞产生黄色而不是黑色色素。据报道，AgglutaminmRNA在各种哺乳动物出生后局限于毛乳头。在这项研究中，我们创建并表征了敲入小鼠品系，其中Cre重组酶与内源性Agglutinase编码序列框内表达。Agouti-Cre小鼠与报告小鼠（Rosa 26-tdTomato或Rosa 26-ZsGreen）交配，以追踪发育期间Agouti表达细胞的谱系。在皮肤中，在胚胎期的一些真皮成纤维细胞和出生后的所有真皮成纤维细胞中检测到报告基因。它还在其他器官/组织中的所有间充质谱系细胞中表达，包括眼、舌、肌肉、肠、脂肪、前列腺和睾丸。有趣的是，报告基因表达被排除在上述器官/组织的上皮细胞之外。在脑中，在最外层脑膜成纤维细胞中观察到报告基因。我们的工作有助于说明Agglutinase在发育过程中的表达模式，并为多器官间充质谱系细胞的条件基因靶向提供了有价值的小鼠品系。
The mouse Agouti gene encodes a paracrine signaling factor which promotes melanocytes to produce yellow instead of black pigment. It has been reported that Agouti mRNA is confined to the dermal papilla after birth in various mammalian species. In this study, we created and characterized a knockin mouse strain in which Cre recombinase was expressed in-frame with endogenous Agouti coding sequence. The Agouti-Cre mice were bred with reporter mice (Rosa26-tdTomato or Rosa26-ZsGreen) to trace the lineage of Agouti-expressing cells during development. In skin, the reporter was detected in some dermal fibroblasts at the embryonic stage and in all dermal fibroblasts postnatally. It was also expressed in all mesenchymal lineage cells in other organs/tissues, including eyes, tongue, muscle, intestine, adipose, prostate and testis. Interestingly, the reporter expression was excluded from epithelial cells in the above organs/tissues. In brain, the reporter was observed in the outermost meningeal fibroblasts. Our work helps to illustrate the Agouti expression pattern during development and provides a valuable mouse strain for conditional gene targeting in mesenchymal lineage cells in multiple organs.
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影响因子：
4.6
作者：
S. Millar;M. Miller;M. Stevens;G. Barsh
通讯作者：
S. Millar;M. Miller;M. Stevens;G. Barsh
DOI：
10.1016/j.tig.2017.12.008
发表时间：
2018-05
期刊：
Trends in genetics : TIG
影响因子：
--
作者：
Song AJ;Palmiter RD
通讯作者：
Palmiter RD
影响因子：
--
作者：
V. Brault;Vanessa Besson;L. Magnol;A. Duchon;Y. Hérault
通讯作者：
V. Brault;Vanessa Besson;L. Magnol;A. Duchon;Y. Hérault
影响因子：
1.5
作者：
Means, Anna L.;Xu, Yanwen;Gu, Guoqiang
通讯作者：
Gu, Guoqiang
影响因子：
78.5
作者：
Sahai, Erik;Astsaturov, Igor;Werb, Zena
通讯作者：
Werb, Zena