The temporal dynamics of Plasmodium species infection after artemisinin-based combination therapy (ACT) among asymptomatic children in the Hohoe municipality, Ghana.
The temporal dynamics of Plasmodium species infection after artemisinin-based combination therapy (ACT) among asymptomatic children in the Hohoe municipality, Ghana.
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DOI：
10.1186/s12936-023-04712-1
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发表时间：
2023-09-14
期刊：
影响因子：
3
通讯作者：
Dinko, Bismarck
中科院分区：
文献类型：
--
作者：
Ansah, Felix;Nyame, Kwamina;Laryea, Rukaya;Owusu, Richard;Amon, Denick;Boyetey, Mark-Jefferson Buer;Ayeke, Dzidzor;Razak, Nasibatu;Kornu, Victor;Ashitei, Sarah;Owusu-Appiah, Caleb;Chirawurah, Jersley;Abugri, James;Aniweh, Yaw;Opoku, Nicholas;Sutherland, Colin;Binka, Fred;Kweku, Margaret;Awandare, Gordon;Dinko, Bismarck
关键词：
使用核酸扩增检测对无症状疟疾进行常规监测，对于获得可靠数据，为制定疟疾政策和执行适当控制措施提供信息至关重要。在这项研究中，在加纳霍霍市内的30个社区的无症状儿童（n = 1697）5岁以下的疟原虫种的患病率和动态进行了测定。聚合酶链反应（PCR）检测疟原虫感染率为33.6%（571/1697），显著高于镜检结果[26.6%（451/1697）]（P < 0.0001）。根据巢式PCR的种特异性分析，恶性疟原虫感染占优势[33.6%（570/1697）]，三日疟原虫感染占0.1%（1/1697），卵形疟原虫感染占0.0%（0/1697），间日疟原虫感染占0.0%（0/1697）。30个社区恶性疟原虫感染率为0.0 ~ 82.5%。在青蒿琥酯-阿莫地喹（AS + AQ，25 mg/kg）治疗的参与者（n = 184）的亚群，有一个大幅度减少疟原虫寄生虫患病率的100%和79.2%，在第7天的基础上显微镜和巢式PCR分析，分别。然而，寄生虫的患病率从第14天到第42天增加，随后在第70天下降，显微镜和巢式PCR。对于寄生虫清除率分析，我们发现显著比例的参与者在开始治疗后第1天[65.0%（13/20）]、第2天[65.0%（13/20）]和第3天[60.0%（12/20）]携带残留疟原虫寄生虫或寄生虫基因组DNA。值得注意的是，治疗前后参与者的配子体携带率较低。综上所述，结果表明，相当数量的个体在治疗后可能携带残留的疟原虫寄生虫或寄生虫基因组DNA。这项研究表明了使用敏感的核酸扩增技术对无症状疟疾进行常规监测的重要性。
The routine surveillance of asymptomatic malaria using nucleic acid-based amplification tests is essential in obtaining reliable data that would inform malaria policy formulation and the implementation of appropriate control measures. In this study, the prevalence rate and the dynamics of Plasmodium species among asymptomatic children (n = 1697) under 5 years from 30 communities within the Hohoe municipality in Ghana were determined. The observed prevalence of Plasmodium parasite infection by polymerase chain reaction (PCR) was 33.6% (571/1697), which was significantly higher compared to that obtained by microscopy [26.6% (451/1697)] (P < 0.0001). Based on species-specific analysis by nested PCR, Plasmodium falciparum infection [33.6% (570/1697)] was dominant, with Plasmodium malariae, Plasmodium ovale and Plasmodium vivax infections accounting for 0.1% (1/1697), 0.0% (0/1697), and 0.0% (0/1697), respectively. The prevalence of P. falciparum infection among the 30 communities ranged from 0.0 to 82.5%. Following artesunate-amodiaquine (AS + AQ, 25 mg/kg) treatment of a sub-population of the participants (n = 184), there was a substantial reduction in Plasmodium parasite prevalence by 100% and 79.2% on day 7 based on microscopy and nested PCR analysis, respectively. However, there was an increase in parasite prevalence from day 14 to day 42, with a subsequent decline on day 70 by both microscopy and nested PCR. For parasite clearance rate analysis, we found a significant proportion of the participants harbouring residual Plasmodium parasites or parasite genomic DNA on day 1 [65.0% (13/20)], day 2 [65.0% (13/20)] and day 3 [60.0% (12/20)] after initiating treatment. Of note, gametocyte carriage among participants was low before and after treatment. Taken together, the results indicate that a significant number of individuals could harbour residual Plasmodium parasites or parasite genomic DNA after treatment. The study demonstrates the importance of routine surveillance of asymptomatic malaria using sensitive nucleic acid-based amplification techniques.
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DOI：
10.1016/j.jmoldx.2021.07.022
发表时间：
2021-10
期刊：
The Journal of molecular diagnostics : JMD
影响因子：
--
作者：
Ansah F;Suurbaar J;Darko D;Anabire NG;Blankson SO;Domson BKS;Soulama A;Kpasra P;Chirawurah JD;Amenga-Etego L;Kanyong P;Awandare GA;Aniweh Y
通讯作者：
Aniweh Y
DOI：
10.1016/s1473-3099(21)00072-4
发表时间：
2021-11
期刊：
The Lancet. Infectious diseases
影响因子：
--
作者：
Andolina C;Rek JC;Briggs J;Okoth J;Musiime A;Ramjith J;Teyssier N;Conrad M;Nankabirwa JI;Lanke K;Rodriguez-Barraquer I;Meerstein-Kessel L;Arinaitwe E;Olwoch P;Rosenthal PJ;Kamya MR;Dorsey G;Greenhouse B;Drakeley C;Staedke SG;Bousema T
通讯作者：
Bousema T
影响因子：
3
作者：
Carlsson, Anja M.;Ngasala, Billy E.;Martensson, Andreas
通讯作者：
Martensson, Andreas
影响因子：
11.8
作者：
Groger, Miriam;Veletzky, Luzia;Ramhartert, Michael
通讯作者：
Ramhartert, Michael
影响因子：
2.4
作者：
Bruce, MC;Donnelly, CA;Day, KP
通讯作者：
Day, KP