Development of Cooperative Primer-Based Real-Time PCR Assays for the Detection of Plasmodium malariae and Plasmodium ovale.
Development of Cooperative Primer-Based Real-Time PCR Assays for the Detection of Plasmodium malariae and Plasmodium ovale.
复制标题
DOI：
10.1016/j.jmoldx.2021.07.022
复制
发表时间：
2021-10
期刊：
影响因子：
--
通讯作者：
Aniweh Y
中科院分区：
文献类型：
--
作者：
Ansah F;Suurbaar J;Darko D;Anabire NG;Blankson SO;Domson BKS;Soulama A;Kpasra P;Chirawurah JD;Amenga-Etego L;Kanyong P;Awandare GA;Aniweh Y
随着恶性疟疾全球传播的减少，三日疟原虫和卵形疟原虫日益引起公众健康的关注。然而，缺乏可靠的疟原虫物种特异性检测工具，阻碍了这些次要疟原虫物种的准确诊断。在这项研究中，SYBR Green为基础的实时PCR检测三日疟原虫和卵形疟原虫使用合作引物，显着限制引物二聚体的形成和繁殖的检测。三日疟原虫和卵形疟原虫基于合作引物的检测与相应的常规基于引物的检测相比具有至少10倍的低检测限。更重要的是，合作的引物为基础的检测进行了评估，在一项横断面研究中使用560个样本从两个卫生设施在加纳。合并研究人群中三日疟原虫和卵形疟原虫的患病率分别为18.6%（104/560）和5.5%（31/560）。在阳性病例中，三日疟原虫和卵形疟原虫分别占3.6%（18/499）和1.0%（5/499），其余均为恶性疟原虫混合感染。该研究表明，在非恶性疟原虫物种的常规诊断和监测中，包括检测限较低的检测工具对公共卫生的重要性。这对于全面评估旨在全球消除疟疾的疟疾干预和控制措施的有效性是必要的。
Plasmodium malariae and Plasmodium ovale are increasingly gaining public health attention as the global transmission of falciparum malaria is decreasing. However, the absence of reliable Plasmodium species-specific detection tools has hampered accurate diagnosis of these minor Plasmodium species. In this study, SYBR Green–based real-time PCR assays were developed for the detection of P. malariae and P. ovale using cooperative primers that significantly limit the formation and propagation of primers-dimers. Both the P. malariae and P. ovale cooperative primer-based assays had at least 10-fold lower detection limit compared with the corresponding conventional primer-based assays. More important, the cooperative primer-based assays were evaluated in a cross-sectional study using 560 samples obtained from two health facilities in Ghana. The prevalence rates of P. malariae and P. ovale among the combined study population were 18.6% (104/560) and 5.5% (31/560), respectively. Among the Plasmodium-positive cases, P. malariae and P. ovale mono-infections were 3.6% (18/499) and 1.0% (5/499), respectively, with the remaining being co-infections with Plasmodium falciparum. The study demonstrates the public health importance of including detection tools with lower detection limits in routine diagnosis and surveillance of nonfalciparum species. This will be necessary for comprehensively assessing the effectiveness of malaria interventions and control measures aimed toward global malaria elimination.
登录
查看更多内容
影响因子：
12
作者：
Erdman, Laura K;Kain, Kevin C
通讯作者：
Kain, Kevin C
影响因子：
11.8
作者：
Lo, Eugenia;Nguyen, Kristie;Yan, Guiyun
通讯作者：
Yan, Guiyun
影响因子：
3.7
作者：
Ayanful-Torgby, Ruth;Quashie, Neils B.;Amoah, Linda E.
通讯作者：
Amoah, Linda E.
影响因子：
3.7
作者：
Camargo-Ayala PA;Cubides JR;Niño CH;Camargo M;Rodríguez-Celis CA;Quiñones T;Sánchez-Suárez L;Patarroyo ME;Patarroyo MA
通讯作者：
Patarroyo MA
影响因子：
3
作者：
Niño CH;Cubides JR;Camargo-Ayala PA;Rodríguez-Celis CA;Quiñones T;Cortés-Castillo MT;Sánchez-Suárez L;Sánchez R;Patarroyo ME;Patarroyo MA
通讯作者：
Patarroyo MA