Stem cells from human-exfoliated deciduous teeth can differentiate into dopaminergic neuron-like cells.
Stem cells from human-exfoliated deciduous teeth can differentiate into dopaminergic neuron-like cells.
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来自人类脱落乳牙的干细胞可以分化为多巴胺能神经元样细胞。
DOI：
10.1089/scd.2009.0258
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发表时间：
2010-09
影响因子：
4
通讯作者：
Wang S
中科院分区：
文献类型：
--
作者：
Wang J;Wang X;Sun Z;Wang X;Yang H;Shi S;Wang S
人脱落乳牙干细胞（SHED）是一种新生的干细胞，具有分化为神经细胞、牙源性细胞和脂肪细胞的能力。据报道，当在神经诱导条件下培养时，基于细胞形态和早期神经元标记物的表达，SHED分化为神经细胞。因此，本研究在大鼠模型中研究了SHED在缓解帕金森病（PD）中的治疗功效。我们发现，SHED可以诱导形成神经样球在培养基中优化神经干细胞在体外。在与包括音刺猬、成纤维细胞生长因子8、神经胶质细胞系衍生的神经营养因子和毛喉素的细胞因子的混合物一起孵育后，这些SHED衍生的球体进一步分化成含有特异性多巴胺能神经元的细胞群。此外，移植到帕金森病大鼠纹状体的SHED球部分改善阿朴吗啡诱发的旋转的神经功能障碍相比，移植控制SHED。我们的数据表明，SHED，可能来自神经嵴细胞，可能是PD治疗的出生后干细胞的最佳来源。
Stem cells from human exfoliated deciduous teeth (SHED) have been identified as a novel population of postnatal stem cells capable of differentiating into neural cells, odontogenic cells, and adipocytes. SHED were reported to differentiate into neural cells based on cellular morphology and the expression of early neuronal markers when cultured under neural inductive conditions. This study therefore investigated the therapeutic efficacy of SHED in alleviating Parkinson's disease (PD) in a rat model. We found that SHED could be induced to form neural-like spheres in a medium optimized for neural stem cells in vitro. After incubation with a cocktail of cytokines including sonic hedgehog, fibroblast growth factor 8, glial cell line-derived neurotrophic factor, and forskolin, these SHED-derived spheres further differentiated into a cell population that contained specific dopaminergic neurons. Moreover, transplantation of SHED spheres into the striatum of parkinsonian rats partially improved the apomorphine-evoked rotation of behavorial disorders compared to transplantation of control SHED. Our data indicate that SHED, potentially derived from neural crest cells, may be an optimal source of postnatal stem cells for PD treatment.
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