A novel in vitro loading system to produce supraphysiologic oscillatory fluid shear stress.
A novel in vitro loading system to produce supraphysiologic oscillatory fluid shear stress.
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DOI：
10.1016/j.jbiomech.2013.10.036
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发表时间：
2014-01-22
影响因子：
2.4
通讯作者：
Mann KA
中科院分区：
文献类型：
--
作者：
Oest ME;Miller MA;Howard KI;Mann KA
开发了一种多孔流体加载（MFL）系统，使用标准多孔培养板在体外将振荡的亚生理至超生理流体剪切应力传递至细胞单层。采用流体-结构相互作用的计算流体动力学模型，对轴向位移活塞和孔板表面之间的挤压膜流体流动进行了定量分析。调整活塞基座的锥角调制流体压力、速度和剪切应力的大小。模拟结果表明，在整个井中存在接近均匀的流体剪切应力，在整个井的半径上具有线性压降。使用MFL系统，将RAW 264.7骨细胞暴露于0、0.5、1.5、4、6和17 Pa的振荡流体剪切应力。以1Hz每天加载细胞1小时，持续两天。与亚生理和生理水平相比，超生理振荡流体剪切诱导骨细胞活性的上调，通过抗酒石酸酸性磷酸酶活性和矿物吸收坑的形成来测量。所有处理组的细胞数量保持恒定。
A multi-well fluid loading (MFL) system was developed to deliver oscillatory subphysiologic to supraphysiologic fluid shear stresses to cell monolayers in vitro using standard multi-well culture plates. Computational fluid dynamics modeling with fluid-structure interactions was used to quantify the squeeze film fluid flow between an axially displaced piston and the well plate surface. Adjusting the cone angle of the piston base modulated the fluid pressure, velocity, and shear stress magnitudes. Modeling results showed that there was near uniform fluid shear stress across the well with a linear drop in pressure across the radius of the well. Using the MFL system, RAW 264.7 osteoclastic cells were exposed to oscillatory fluid shear stresses of 0, 0.5, 1.5, 4, 6, and 17 Pa. Cells were loaded 1 h per day at 1 Hz for two days. Compared to sub-physiologic and physiologic levels, supraphysiologic oscillatory fluid shear induced upregulation of osteoclastic activity as measured by tartrate-resistant acid phosphatase activity and formation of mineral resorption pits. Cell number remained constant across all treatment groups.
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影响因子：
2.4
作者：
Cardoso L;Fritton SP;Gailani G;Benalla M;Cowin SC
通讯作者：
Cowin SC
影响因子：
2.8
作者：
Case, Natasha;Xie, Zhihui;Sen, Buer;Styner, Maya;Zou, Minxu;O'Conor, Chris;Horowitz, Mark;Rubin, Janet
通讯作者：
Rubin, Janet
影响因子：
2.4
作者：
Anderson, Eric J.;Tate, Melissa L. Knothe
通讯作者：
Tate, Melissa L. Knothe
DOI：
10.1302/0301-620x.82b5.9561
发表时间：
2000-07-01
影响因子：
--
作者：
Ferrier, GM;McEvoy, A;Andrew, JG
通讯作者：
Andrew, JG
影响因子：
4.1
作者：
McEvoy, A;Jeyam, M;Andrew, JG
通讯作者：
Andrew, JG