Modification of chrysanthemum odour and taste with chrysanthemol synthase induces strong dual resistance against cotton aphids.
Modification of chrysanthemum odour and taste with chrysanthemol synthase induces strong dual resistance against cotton aphids.
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菊酚合酶修饰菊花气味和味道可诱导对棉蚜的强烈双重抗性
DOI：
10.1111/pbi.12885
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发表时间：
2018-08
影响因子：
13.8
通讯作者：
Wang C
中科院分区：
文献类型：
--
作者：
Hu H;Li J;Delatte T;Vervoort J;Gao L;Verstappen F;Xiong W;Gan J;Jongsma MA;Wang C
摘要蚜虫是菊花的害虫，在长时间取食和繁殖之前，利用植物挥发物选择宿主植物并摄取细胞内容物以探测宿主质量。挥发性和非挥发性代谢物谱的变化可以破坏蚜虫-植物相互作用，并提供新的害虫控制方法。菊醇合成酶（CHS）是除虫菊酯类杀虫剂生物合成的第一步，但尚没有单独的菊醇产物的生物学作用。在这项研究中，TcCHS基因在杭白菊中过表达，导致挥发性胆固醇（约100 mg/kg）的释放。47 pmol/h/gFW）和胆甾醇糖苷衍生物（通过NMR鉴定为胆甾醇-6-O-丙二酰基-β-D-吡喃葡萄糖苷）的蓄积（约1.1 mM），没有有害的表型效应。分别测定这些化合物的纯品形式和作为顶空和提取物的一部分的双重选择测定证明了两种组分对棉蚜（Aphis gossypii）的独立生物活性。性能测定表明，TcCHS植物显着减少蚜虫繁殖，与蚜虫探测活动的干扰，这些植物上所揭示的脑电图（EPG）的研究一致。在开放田间试验中，蚜虫种群发育受到非常严重的损害，证明了该性状的稳健性和高影响。结果表明，TcCHS基因的表达诱导了一个双重防御系统，具有胆甾醇气味的驱避性和其非挥发性糖苷的威慑力，为植物工程蚜虫控制引入了一个有前途的新选择。
Summary Aphids are pests of chrysanthemum that employ plant volatiles to select host plants and ingest cell contents to probe host quality before engaging in prolonged feeding and reproduction. Changes in volatile and nonvolatile metabolite profiles can disrupt aphid–plant interactions and provide new methods of pest control. Chrysanthemol synthase (CHS) from Tanacetum cinerariifolium represents the first committed step in the biosynthesis of pyrethrin ester insecticides, but no biological role for the chrysanthemol product alone has yet been documented. In this study, the TcCHS gene was over‐expressed in Chrysanthemum morifolium and resulted in both the emission of volatile chrysanthemol (ca. 47 pmol/h/gFW) and accumulation of a chrysanthemol glycoside derivative, identified by NMR as chrysanthemyl‐6‐O‐malonyl‐β‐D‐glucopyranoside (ca. 1.1 mM), with no detrimental phenotypic effects. Dual‐choice assays separately assaying these compounds in pure form and as part of the headspace and extract demonstrated independent bioactivity of both components against the cotton aphid (Aphis gossypii). Performance assays showed that the TcCHS plants significantly reduced aphid reproduction, consistent with disturbance of aphid probing activities on these plants as revealed by electropenetrogram (EPG) studies. In open‐field trials, aphid population development was very strongly impaired demonstrating the robustness and high impact of the trait. The results suggest that expression of the TcCHS gene induces a dual defence system, with both repellence by chrysanthemol odour and deterrence by its nonvolatile glycoside, introducing a promising new option for engineering aphid control into plants.
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影响因子：
2.6
作者：
Costa, Rosaria;De Fina, Maria Rosa;Mondello, Luigi
通讯作者：
Mondello, Luigi
影响因子：
2.6
作者：
Halbert, S. E.;Corsini, D.;Vaughn, S. F.
通讯作者：
Vaughn, S. F.
影响因子：
10.4
作者：
Barr DB;Olsson AO;Wong LY;Udunka S;Baker SE;Whitehead RD;Magsumbol MS;Williams BL;Needham LL
通讯作者：
Needham LL
DOI：
10.1073/pnas.0603998103
发表时间：
2006-07-05
影响因子：
11.1
作者：
Beale, Michael H.;Birkett, Michael A.;Zhang, Yuhua
通讯作者：
Zhang, Yuhua
影响因子：
1.6
作者：
Kim, Yun-Soo;Lim, Soon;Sano, Hiroshi
通讯作者：
Sano, Hiroshi