Comparative RNA-seq based transcriptome profiling of waterlogging response in cucumber hypocotyls reveals novel insights into the de novo adventitious root primordia initiation.
Comparative RNA-seq based transcriptome profiling of waterlogging response in cucumber hypocotyls reveals novel insights into the de novo adventitious root primordia initiation.
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基于RNA-seq的黄瓜下胚轴浸水反应转录组分析揭示了不定根原基从头启动的新见解
DOI：
10.1186/s12870-017-1081-8
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发表时间：
2017-07-26
影响因子：
5.3
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Xu X;Chen M;Ji J;Xu Q;Qi X;Chen X
研究背景涝渍是一种严重影响植物生长的非生物胁迫，它导致植物体内组织供氧能力下降。虽然黄瓜（Cucumis sativusL.）对水涝敏感，其产生不定根（ARs）的能力有利于气体扩散，并在氧气浓度降低时增加植物存活。为了更好地理解淹水条件下AR原基重新出现的分子机制，本研究采用基于RNA测序的转录组学方法，对两种不同的黄瓜基因型早二-N（耐涝）和Pepino结果在黄瓜下胚轴中检测到27,000多个转录本，其中，在‘早二-N’和‘Pepino’中分别有1494和1766个基因在淹水后2天差异表达。RNA测序数据与qPCR分析之间的显著正相关表明所鉴定的基因是可信的。比较分析表明，在碳水化合物动员，硝酸盐同化，激素产生和信号通路，转录因子和细胞分裂的基因功能可能有助于水浸触发AR原基的启动。乙烯被确定为负责黄瓜AR启动的重要植物激素。此外，编码细胞色素P450，锚蛋白重复蛋白和亚硫酸盐氧化酶的基因被确定为重要的渍aculations.ConclusionThis研究拓宽了我们的理解的机制，潜在的渍引发AR的出现，并提供了有价值的信息，为黄瓜耐渍性增强的育种。
BackgroundWaterlogging is a serious abiotic stress to plant growth because it results in the decline in the supplement of oxygen to submerged tissues. Although cucumber (Cucumis sativusL.) is sensitive to waterlogging, its ability to generate adventitious roots (ARs) facilitates gas diffusion and increases plant survival when the oxygen concentration is decreased. To gain a better understanding of the molecular mechanisms that enable de novo AR primordia emergence upon waterlogging, the RNA sequencing-based transcriptomic responses of two contrasting cucumber genotypes, Zaoer-N (waterlogging tolerant) and Pepino (waterlogging sensitive), which differed in their abilities to form AR were compared.ResultsMore than 27,000 transcripts were detected in cucumber hypocotyls, from which 1494 and 1766 genes in ‘Zaoer-N’ and ‘Pepino’, respectively, were differentially expressed 2 days after waterlogging. The significant positive correlation between RNA sequencing data and a qPCR analysis indicated that the identified genes were credible. A comparative analysis revealed that genes functioning in carbohydrate mobilization, nitrate assimilation, hormone production and signaling pathways, transcription factors and cell division might contribute to the waterlogging-triggered AR primordia initiation. Ethylene was determined to be an important plant hormone responsible for the cucumber ARs initiation. Additionally, genes encoding cytochrome P450, ankyrin repeat-containing proteins and sulfite oxidases were determined as important in waterlogging acclimation.ConclusionThis research broadens our understanding of the mechanism underlying waterlogging-triggered ARs emergence, and provides valuable information for the breeding of cucumber with enhanced waterlogging tolerance.
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影响因子：
64.8
作者：
Blilou, I;Xu, J;Scheres, B
通讯作者：
Scheres, B
影响因子：
4.4
作者：
Abu-Abied M;Szwerdszarf D;Mordehaev I;Yaniv Y;Levinkron S;Rubinstein M;Riov J;Ophir R;Sadot E
通讯作者：
Sadot E
影响因子：
5.2
作者：
Kumutha, D.;Sairam, R. K.;Meena, R. C.
通讯作者：
Meena, R. C.
影响因子：
4.2
作者：
BLOM, CWPM;VOESENEK, LACJ;VISSER, EJW
通讯作者：
VISSER, EJW
影响因子：
1.8
作者：
ARMSTRONG, W;JUSTIN, SHFW;LYTHE, S
通讯作者：
LYTHE, S