IncHI1 plasmids are epidemic vectors that mediate transmission of tet(X4) in Escherichia coli isolated from China.
IncHI1 plasmids are epidemic vectors that mediate transmission of tet(X4) in Escherichia coli isolated from China.
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DOI：
10.3389/fmicb.2023.1153139
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发表时间：
2023
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
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作者：
本研究旨在探讨大肠杆菌中广泛传播泰特（X4）Q6的遗传因素，并描述泰特（X4）的遗传背景。我们分离出E. 2020年，在中国一个大型养鸡场收集的粪便、水、土壤和苍蝇中发现了大肠杆菌。药敏试验和PFGE分型用于确定替加环素耐药性和评估分离株之间的克隆关系。通过接合、S1脉冲场凝胶电泳（PFGE）、质粒稳定性测试和全基因组测序分析质粒存在和基因组序列。共筛选到204株抗替格列汀E.共分离出662株大肠杆菌。其中，我们鉴定了165泰特（X4）的大肠杆菌。这些菌株表现出高度的多药耐药性。根据采样区的地理位置分布、各采样区的样本数以及替加氯啉耐药菌株和泰特（X4）阳性菌株的分离率，筛选出72株泰特（X4）阳性菌株进行进一步调查。72株替加环素耐药菌株具有移动的性，并检出3种携带泰特（X4）质粒的菌株，分别为IncHI 1（n = 67）、IncX 1（n = 3）和pO 111-like/IncFIA（HI 1）（n = 2）。pO 111-like/IncFIA（HI 1）是一种能够转移泰特（X4）的新型质粒。在大多数情况下，IncHI 1质粒的转移效率非常高，并且IncHI 1质粒在转移到普通受体菌株中时是稳定的。含泰特（X4）的IS 1、IS 26和ISCR 2基因两侧的遗传结构复杂，在不同的质粒中存在差异。抗替格列汀E.大肠杆菌是公众健康的主要威胁。该数据表明，在农场谨慎使用四环素对限制替加环素耐药性的传播很重要。携带泰特（X4）的多个移动的元件与IncHI 1质粒循环，IncHI 1质粒是该环境中的优势载体。
This study aimed to investigate the genetic factors promoting widespread Q6 dissemination of tet(X4) between Escherichia coli and to characterize the genetic contexts of tet(X4). We isolated E. coli from feces, water, soil and flies collected across a large-scale chicken farm in China in 2020. Antimicrobial susceptibility testing and PFGE typing were used to identify tigecycline resistance and assess clonal relationships among isolates. Plasmids present and genome sequences were analyzed by conjugation, S1 pulsed-field gel electrophoresis (PFGE), plasmid stability testing and whole-genome sequencing. A total of 204 tigecycline-resistant E. coli were isolated from 662 samples. Of these, we identified 165 tet(X4)-carrying E. coli and these strains exhibited a high degree of multidrug resistance. Based on the geographical location distribution of the sampled areas, number of samples in each area and isolation rate of tigecycline-resistant strains and tet(X4)-carrying isolates, 72 tet(X4)-positive isolates were selected for further investigation. Tigecycline resistance was shown to be mobile in 72 isolates and three types of tet(X4)-carrying plasmids were identified, they were IncHI1 (n = 67), IncX1 (n = 3) and pO111-like/IncFIA(HI1) (n = 2). The pO111-like/IncFIA(HI1) is a novel plasmid capable of transferring tet(X4). The transfer efficiency of IncHI1 plasmids was extremely high in most cases and IncHI1 plasmids were stable when transferred into common recipient strains. The genetic structures flanked by IS1, IS26 and ISCR2 containing tet(X4) were complex and varied in different plasmids. The widespread dissemination of tigecycline-resistant E. coli is a major threat to public health. This data suggests careful use of tetracycline on farms is important to limit spread of resistance to tigecycline. Multiple mobile elements carrying tet(X4) are in circulation with IncHI1 plasmids the dominant vector in this setting.
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影响因子：
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作者：
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通讯作者：
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