Ultra-high-frequency radio-frequency acoustic molecular imaging with saline nanodroplets in living subjects.
Ultra-high-frequency radio-frequency acoustic molecular imaging with saline nanodroplets in living subjects.
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活体中使用盐水纳米液滴的超高频射频声学分子成像。
DOI：
10.1038/s41565-021-00869-5
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发表时间：
2021-06
影响因子：
38.3
通讯作者：
Gambhir SS
中科院分区：
文献类型：
--
作者：
Chen YS;Zhao Y;Beinat C;Zlitni A;Hsu EC;Chen DH;Achterberg F;Wang H;Stoyanova T;Dionne J;Gambhir SS
分子成像是临床诊断中的关键技术，但它依赖于放射性示踪剂或高磁场，这对许多患者，特别是婴儿和孕妇不利。使用非电离RF脉冲的超高频射频声学（UHF-RF-声学）成像允许以亚毫米空间分辨率进行深层组织成像。然而，缺乏生物相容性和靶向造影剂，阻碍了UHF-RF-声学成像在体内的成功应用。在这里，我们报告了我们的发展目标nanodrops的UHF-RF声学分子成像的癌症。我们合成了含有高渗盐水的全液体纳米液滴，其稳定至少2周，并且可以产生高强度的UHF-RF-声学信号。与浓度匹配的氧化铁纳米颗粒相比，我们的纳米液滴在相同的成像深度下产生至少1600倍的UHF-RF-声学信号。我们证明了在表达胃泌素释放蛋白受体（GRPR）的前列腺癌异种移植小鼠模型中使用靶向纳米滴的体内成像，与不表达GRPR的非靶向纳米滴或前列腺癌细胞相比，显示出超过两倍的靶向特异性。
Molecular imaging is a crucial technique in clinical diagnostics, but it relies on radioactive tracers or high magnetic fields that are unfavorable for many patients, particularly infants and pregnant women. Ultra-high-frequency-radiofrequency-acoustic (UHF-RF-acoustic) imaging using non-ionizing RF pulses allows deep-tissue imaging with sub-millimeter spatial resolution. However, lack of biocompatible and targetable contrast agents has prevented the successful in vivo application of UHF-RF-acoustic imaging. Here, we report our development of targetable nanodroplets for UHF-RF-acoustic molecular imaging of cancers. We synthesize all-liquid nanodroplets containing hypertonic saline that are stable for at least 2 weeks and can produce high intensity UHF-RF-acoustic signals. Compared with concentration-matched iron-oxide nanoparticles, our nanodroplets produce at least 1600 times higher UHF-RF-acoustic signals at the same imaging depth. We demonstrate in vivo imaging using the targeted nanodroplets in a prostate cancer xenograft mouse model expressing gastrin release protein receptor (GRPR), showing targeting specificity by more than two-fold, compared to untargeted nanodroplets or prostate cancer cells not expressing GRPR.
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影响因子：
2
作者：
HILDER, MH
通讯作者：
HILDER, MH
影响因子：
16.1
作者：
Hussain, Timon;Nguyen, Quyen T.
通讯作者：
Nguyen, Quyen T.
影响因子：
19.7
作者：
Kruger, RA;Miller, KD;Kruger, GA
通讯作者：
Kruger, GA
影响因子：
17.1
作者：
Gambhir SS;Ge TJ;Vermesh O;Spitler R
通讯作者：
Spitler R
影响因子：
3.8
作者：
Doinikov, Alexander A.;Sheeran, Paul S.;Dayton, Paul A.
通讯作者：
Dayton, Paul A.