The GRAS gene family and its roles in seed development in litchi (Litchi chinensis Sonn).
The GRAS gene family and its roles in seed development in litchi (Litchi chinensis Sonn).
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GRAS基因家族及其在荔枝种子发育中的作用
DOI：
10.1186/s12870-021-03193-1
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发表时间：
2021-09-17
影响因子：
5.3
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Chen J;Yan Q;Li J;Feng L;Zhang Y;Xu J;Xia R;Zeng Z;Liu Y
GRAS基因家族在包括种子发育在内的植物生长过程中起着重要作用，与荔枝无核性状密切相关。然而，在荔枝这一亚热带地区栽培的经济果树中，尚未对其进行充分的鉴定和分析。在这项研究中，48个LcGRAS蛋白被鉴定，并根据其染色体位置命名。通过系统发育分析，LcGRAS蛋白可分为14个亚家族。基因结构和保守结构域分析表明，不同的亚家族具有不同的基序模式，表明其功能的多样性。同线性分析表明，荔枝GRAS家族的扩展可能是由串联和片段重复驱动的。在综合分析degradome数据后，我们发现HAM亚家族的4个LcGRAS基因通过miR 171介导的降解进行调控。LcGRAS基因在不同组织中的不同表达模式揭示了它们参与不同的生物学过程。此外，LcGRAS基因在败育种子和加粗种子中的不同时间表达谱表明，其中一些基因参与维持种子的正常发育。本研究对荔枝GRAS家族成员进行了全面的分析，深入了解了GRAS在荔枝发育中的作用，为进一步研究荔枝种子发育奠定了基础。在线版本包含补充材料，可通过10.1186/s12870-021-03193-1获得。
The GRAS gene family plays crucial roles in multiple biological processes of plant growth, including seed development, which is related to seedless traits of litchi (Litchi chinensis Sonn.). However, it hasn’t been fully identified and analyzed in litchi, an economic fruit tree cultivated in subtropical regions. In this study, 48 LcGRAS proteins were identified and termed according to their chromosomal location. LcGRAS proteins can be categorized into 14 subfamilies through phylogenetic analysis. Gene structure and conserved domain analysis revealed that different subfamilies harbored various motif patterns, suggesting their functional diversity. Synteny analysis revealed that the expansion of the GRAS family in litchi may be driven by their tandem and segmental duplication. After comprehensively analysing degradome data, we found that four LcGRAS genes belong to HAM subfamily were regulated via miR171-mediated degradation. The various expression patterns of LcGRAS genes in different tissues uncovered they were involved in different biological processes. Moreover, the different temporal expression profiles of LcGRAS genes between abortive and bold seed indicated some of them were involved in maintaining the normal development of the seed. Our study provides comprehensive analyses on GRAS family members in litchi, insight into a better understanding of the roles of GRAS in litchi development, and lays the foundation for further investigations on litchi seed development. The online version contains supplementary material available at 10.1186/s12870-021-03193-1.
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