The GRAS gene family in pine: transcript expression patterns associated with the maturation-related decline of competence to form adventitious roots.
The GRAS gene family in pine: transcript expression patterns associated with the maturation-related decline of competence to form adventitious roots.
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DOI：
10.1186/s12870-014-0354-8
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发表时间：
2014-12-30
影响因子：
5.3
通讯作者：
Díaz-Sala C
中科院分区：
文献类型：
--
作者：
Abarca D;Pizarro A;Hernández I;Sánchez C;Solana SP;Del Amo A;Carneros E;Díaz-Sala C
不定根是一种器官发生的过程，通过它可以从分化的细胞中诱导出根，而不是那些特定发育根的细胞。在森林树种中，年龄和成熟度是茎插条形成不定根的障碍。作为不定根形成基础的完全分化的祖细胞重新具体化背后的机制尚不清楚。在这里，描述了松树GRAS基因家族的特征，并报道了这些基因的一个子集在不定根过程中的表达。蛋白质结构的比较分析表明，与其近亲相比，被子植物中的松树成员是保守的。在未分化的增殖性胚性培养和胚胎发育过程中，检测到相对较高的Gras基因表达水平。在不再具有不定根形成能力的成体组织中，可能的GRAS家族转录因子，包括辐射松稻草人(Scr)、PrSCR和稻草人样(SCL)6、PrSCL6的mRNA水平显著降低或不存在，但在有生根能力的成体组织中被重新编程后保持或诱导。短根(PrSHR)、PrSCL1、PrSCL2、PrSCL10和PrSCL12基因在不定根形成过程中也以生长素、年龄或发育相关的方式表达。松树GRAS家族通过分析蛋白质结构、系统发育关系、保守基序和基因表达模式等方面进行了研究。每一组中的单个基因都具有不同的特殊功能，其中一些可能与成体细胞形成不定根的能力和重新编程有关。本文的在线版本(doi：10.1186/s12870-0140354-8)包含补充材料，授权用户可以使用。
Adventitious rooting is an organogenic process by which roots are induced from differentiated cells other than those specified to develop roots. In forest tree species, age and maturation are barriers to adventitious root formation by stem cuttings. The mechanisms behind the respecification of fully differentiated progenitor cells, which underlies adventitious root formation, are unknown. Here, the GRAS gene family in pine is characterized and the expression of a subset of these genes during adventitious rooting is reported. Comparative analyses of protein structures showed that pine GRAS members are conserved compared with their relatives in angiosperms. Relatively high GRAS mRNA levels were measured in non-differentiated proliferating embryogenic cultures and during embryo development. The mRNA levels of putative GRAS family transcription factors, including Pinus radiata’s SCARECROW (SCR), PrSCR, and SCARECROW-LIKE (SCL) 6, PrSCL6, were significantly reduced or non-existent in adult tissues that no longer had the capacity to form adventitious roots, but were maintained or induced after the reprogramming of adult cells in rooting-competent tissues. A subset of genes, SHORT-ROOT (PrSHR), PrSCL1, PrSCL2, PrSCL10 and PrSCL12, was also expressed in an auxin-, age- or developmental-dependent manner during adventitious root formation. The GRAS family of pine has been characterized by analyzing protein structures, phylogenetic relationships, conserved motifs and gene expression patterns. Individual genes within each group have acquired different and specialized functions, some of which could be related to the competence and reprogramming of adult cells to form adventitious roots. The online version of this article (doi:10.1186/s12870-014-0354-8) contains supplementary material, which is available to authorized users.
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影响因子：
6.9
作者：
Elhiti M;Tahir M;Gulden RH;Khamiss K;Stasolla C
通讯作者：
Stasolla C
影响因子：
6.9
作者：
Duval I;Lachance D;Giguère I;Bomal C;Morency MJ;Pelletier G;Boyle B;MacKay JJ;Séguin A
通讯作者：
Séguin A
影响因子：
2.9
作者：
Abarca, Dolores;Diaz-Sala, Carmen
通讯作者：
Diaz-Sala, Carmen
影响因子：
6.4
作者：
Díaz-Sala, C;Garrido, G;Sabater, B
通讯作者：
Sabater, B
影响因子：
5.1
作者：
Day, RB;Tanabe, S;Minami, E
通讯作者：
Minami, E