Influence of basement membrane proteins and endothelial cell-derived factors on the morphology of human fetal-derived astrocytes in 2D.
Influence of basement membrane proteins and endothelial cell-derived factors on the morphology of human fetal-derived astrocytes in 2D.
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DOI：
10.1371/journal.pone.0092165
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Searson PC
中科院分区：
文献类型：
--
作者：
Levy AF;Zayats M;Guerrero-Cazares H;Quiñones-Hinojosa A;Searson PC
星形胶质细胞是大脑中最常见的胶质细胞类型，参与从调节脑血流到控制突触形成的各种不同功能。星形胶质细胞和星形胶质细胞条件培养液被广泛应用于血脑屏障(BBB)模型中，然而，关于星形胶质细胞二维培养的研究很少。为了验证表面涂层和可溶性因素影响星形胶质细胞二维形态的假设，我们定量分析了人胎儿星形胶质细胞在玻璃、基质、纤维连接蛋白、IV型胶原和I型胶原上的形态，以及加入可溶性因子包括血小板衍生生长因子(PDGF)、层粘连蛋白、碱性成纤维细胞生长因子(BFGF)和白血病抑制因子(LIF)后的形态。Matrigel表面涂层以及在培养液中添加白血病抑制因子(LIF)被发现对2D星形胶质细胞的形态有最强的影响，并可能在改进现有的BBB模型中发挥重要作用。此外，本文提出的一组新的定量参数为确定化合物对星形胶质细胞形态的影响提供了一种测试，既可以筛选影响星形胶质细胞的新的内皮细胞分泌因子，也可以高通量地确定哪些因素对于转换到更复杂的3D BBB模型是重要的。
Astrocytes are the most prevalent type of glial cell in the brain, participating in a variety of diverse functions from regulating cerebral blood flow to controlling synapse formation. Astrocytes and astrocyte-conditioned media are widely used in models of the blood-brain barrier (BBB), however, very little is known about astrocyte culture in 2D. To test the hypothesis that surface coating and soluble factors influence astrocyte morphology in 2D, we quantitatively analyzed the morphology of human fetal derived astrocytes on glass, matrigel, fibronectin, collagen IV, and collagen I, and after the addition soluble factors including platelet-derived growth factor (PDGF), laminin, basic fibroblast growth factor (bFGF), and leukemia inhibitory factor (LIF). Matrigel surface coatings, as well as addition of leukemia inhibitory factor (LIF) to the media, were found to have the strongest effects on 2D astrocyte morphology, and may be important in improving existing BBB models. In addition, the novel set of quantitative parameters proposed in this paper provide a test for determining the influence of compounds on astrocyte morphology, both to screen for new endothelial cell-secreted factors that influence astrocytes, and to determine in a high-throughput way which factors are important for translation to more complex, 3D BBB models.
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DOI：
10.1523/jneurosci.4707-08.2009
发表时间：
2009-03-11
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Oberheim NA;Takano T;Han X;He W;Lin JH;Wang F;Xu Q;Wyatt JD;Pilcher W;Ojemann JG;Ransom BR;Goldman SA;Nedergaard M
通讯作者：
Nedergaard M
影响因子：
3.1
作者：
Nizet, V;Kim, KS;Rubens, CE
通讯作者：
Rubens, CE
影响因子：
2.9
作者：
Siddharthan, Venkatraman;Kim, Yuri V.;Kim, Kwang Sik
通讯作者：
Kim, Kwang Sik
影响因子：
64.8
作者：
NOBLE, M;MURRAY, K;RIDDLE, P
通讯作者：
RIDDLE, P
DOI：
10.1016/j.ijdevneu.2009.07.006
发表时间：
2009-11-01
影响因子：
1.8
作者：
Fukushima, Mikiko;Setoguchi, Takao;Taga, Tetsuya
通讯作者：
Taga, Tetsuya