Cyanobacterial community diversity in the sediments of the Pearl River Estuary in China

Cyanobacterial community diversity in the sediments of the Pearl River Estuary in China
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中国珠江口沉积物中蓝藻群落多样性

DOI:
10.3989/scimar.04106.07a
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发表时间:
2017-12
期刊:
影响因子:
1.4
通讯作者:
Sun Cui-Ci
Sun Cui-Ci
中科院分区:
生物学4区
文献类型:
--
作者:
Sun Fu-Lin;Wang You-Shao;Wu Mei-Lin;Sun Cui-Ci

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采用变性梯度凝胶电泳(DGGE)技术,对珠江口潮季和枯季沉积物中的蓝藻群落多样性进行了研究。DGGE条带的核苷酸序列将蓝藻分为聚球藻属(Synechococcus)、蓝藻属(Cyanobium)、色球藻属(Chroococcus)、前绿藻目(Prochlorales)和单歧藻属(Tolypothrix)5个类群。聚球藻被确定为优势蓝藻组的沉积物样品中,其分布从内河口到外河口,具有广泛的盐度适应。观察到的模式的蓝藻群落之间的采样点和季节变化显着,这表明大多数蓝藻没有通过淡水。典范对应分析,以确定在旱季的环境变量和细菌群落结构之间的关系。结果表明,pH、盐度、PO 4 -P和NO3-N对沉积物中蓝藻群落有显著影响.
Cyanobacterial community diversity in the sediment of the Pearl River Estuary in China was evaluated in this study by denaturing gradient gel electrophoresis (DGGE) during the wet and dry seasons. Nucleotide sequences obtained from DGGE bands were classified into five cyanobacterial clusters, including Synechococcus, Cyanobium, Chroococcus, Prochlorales and Tolypothrix. Synechococcus was identified as the dominant cyanobacterial group in the sediment samples; its distribution varied from the inner estuary to the outer estuary, with a wide range of salinity adaptation. Observed patterns of cyanobacterial communities changed markedly between sampling sites and seasons, suggesting that most cyanobacteria were not delivered via fresh water. Canonical correspondence analysis was conducted to determine the relationship between environmental variables and bacterial community structures during the dry season. The results suggested that the cyanobacterial community was significantly influenced by pH, salinity, PO 4 -P and NO 3 -N in sediments.
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