Gel permeation chromatography of asymmetric proteins.
Gel permeation chromatography of asymmetric proteins.
复制标题
不对称蛋白质的凝胶渗透色谱。
DOI：
10.1016/0003-2697(82)90474-2
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发表时间：
1982
影响因子：
2.9
通讯作者：
Nathans,GR
中科院分区：
文献类型：
--
作者：
Meredith,SC;Nathans,GR
The gel permeation chromatographic behavior of three asymmetric proteins—collagen, fibrinogen, and the prolate ellipsoid lysozyme—was investigated using a variety of gel and high-performance liquid chromatographic media of various pore sizes and a wide range of flow rates. The time dependency of the elution patterns for columns and the partitioning of proteins between solvent and gel phases in batch experiments show that the “anomalous” behavior of asymmetric proteins is explicable by the mechanism proposed by Y. Nozaki, N. M. Schechter, J. A. Reynolds, and C. Tanford (1976, Biochemistry15, 3884); i.e., that these proteins penetrate pores of a size comparable to the minor semiaxis of the protein by end-on insertion. Thus, native type I collagen behaves as if it were a spherical protein of radius 8.2 Å, fibrinogen has an apparent radius of 32.4 Å, and lysozyme has an apparent radius of 14.6 Å. The rate at which asymmetric proteins penetrate the gel interior, however, is slow compared to the rate of gel penetration by globular proteins. The end-on insertion mechanism predicts that given infinite time, asymmetric proteins will be included into that portion of the internal volume of the gel which their smallest projectional cross sections allow them to penetrate. A method is presented for extrapolating the elution volume of asymmetric proteins to infinitely slow flow rate; from this extrapolation, one can calculate the minor semiaxis of the protein.
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牛乳黄嘌呤氧化酶：通过超滤和省略添加蛋白酶的常规方法进行纯化：天然黄嘌呤氧化酶同质性的一些标准。
DOI：
10.1016/0005-2744(78)90125-0
发表时间：
1978
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
G. R. Nathans;E. Hade
通讯作者：
E. Hade
使用凝胶色谱法测定存在和不存在去污剂时蛋白质的斯托克斯半径。
影响因子：
2.9
作者：
Y. Nozaki;N. Schechter;J. Reynolds;C. Tanford
通讯作者：
C. Tanford
通过凝胶电泳技术评估分子大小。
DOI：
10.1515/bchm2.1974.355.2.1281
发表时间：
1974
期刊：
Hoppe-Seyler's Zeitschrift fur physiologische Chemie
影响因子：
--
作者：
K. Felgenhauer
通讯作者：
K. Felgenhauer
影响因子：
1.1
作者：
COLVIN, JR
通讯作者：
COLVIN, JR
DOI：
10.1016/0005-2744(78)90045-1
发表时间：
1978-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
FERNANDEZSOUSA, JM;PEREZCASTELLS, R;RODRIGUEZ, R
通讯作者：
RODRIGUEZ, R